TY - JOUR
T1 - Biomarkers of one-carbon metabolism are associated with biomarkers of inflammation in women
AU - Abbenhardt, Clare
AU - Miller, Joshua W.
AU - Song, Xiaoling
AU - Brown, Elissa C.
AU - Cheng, Ting Yuan David
AU - Wener, Mark H.
AU - Zheng, Yingye
AU - Toriola, Adetunji T.
AU - Neuhouser, Marian L.
AU - Beresford, Shirley A.A.
AU - Makar, Karen W.
AU - Bailey, Lynn B.
AU - Maneval, David R.
AU - Green, Ralph
AU - Manson, Jo Ann E.
AU - Van Horn, Linda
AU - Ulrich, Cornelia M.
PY - 2014/5
Y1 - 2014/5
N2 - Folate-mediated one-carbon metabolism is essential for DNA synthesis, repair, and methylation. Perturbations in onecarbon metabolism have been implicated in increased risk of some cancers and may also affect inflammatory processes. We investigated these interrelated pathways to understand their relation. The objective was to explore associations between inflammation and biomarkers of nutritional status and one-carbon metabolism. In a cross-sectional study in 1976 women selected from the Women's Health Initiative Observational Study, plasma vitamin B-6 [pyridoxal-5'-phosphate (PLP)], plasma vitamin B-12, plasma folate, and RBC folate were measured as nutritional biomarkers; serum C-reactive protein (CRP) and serum amyloid A (SAA) were measured as biomarkers of inflammation; and homocysteine and cysteine were measured as integrated biomarkers of one-carbon metabolism. Student's t, chi-square, and Spearman rank correlations, along with multiple linear regressions, were used to explore relations between biomarkers; additionally, we tested stratification by folic acid fortification period and multivitamin use. With the use of univariate analysis, plasma PLP was the only nutritional biomarker that was modestly significantly correlated with serum CRP and SAA (r = 20.22 and 20.12, respectively; P < 0.0001). Homocysteine (mmol/L) showed significant inverse correlations with all nutritional biomarkers (ranging from r = 20.30 to r = 20.46; all P < 0.0001). With the use of multiple linear regression, plasma PLP, RBC folate, homocysteine, and cysteine were identified as independent predictors of CRP; and PLP, vitamin B-12, RBC folate, and homocysteine were identified as predictors of SAA. When stratified by folic acid fortification period, nutritionhomocysteine correlations were generally weaker in the postfortification period, whereas associations between plasma PLP and serum CRP increased. Biomarkers of inflammation are associated with PLP, RBC folate, and homocysteine in women. The connection between the pathways needs to be further investigated and causality established.
AB - Folate-mediated one-carbon metabolism is essential for DNA synthesis, repair, and methylation. Perturbations in onecarbon metabolism have been implicated in increased risk of some cancers and may also affect inflammatory processes. We investigated these interrelated pathways to understand their relation. The objective was to explore associations between inflammation and biomarkers of nutritional status and one-carbon metabolism. In a cross-sectional study in 1976 women selected from the Women's Health Initiative Observational Study, plasma vitamin B-6 [pyridoxal-5'-phosphate (PLP)], plasma vitamin B-12, plasma folate, and RBC folate were measured as nutritional biomarkers; serum C-reactive protein (CRP) and serum amyloid A (SAA) were measured as biomarkers of inflammation; and homocysteine and cysteine were measured as integrated biomarkers of one-carbon metabolism. Student's t, chi-square, and Spearman rank correlations, along with multiple linear regressions, were used to explore relations between biomarkers; additionally, we tested stratification by folic acid fortification period and multivitamin use. With the use of univariate analysis, plasma PLP was the only nutritional biomarker that was modestly significantly correlated with serum CRP and SAA (r = 20.22 and 20.12, respectively; P < 0.0001). Homocysteine (mmol/L) showed significant inverse correlations with all nutritional biomarkers (ranging from r = 20.30 to r = 20.46; all P < 0.0001). With the use of multiple linear regression, plasma PLP, RBC folate, homocysteine, and cysteine were identified as independent predictors of CRP; and PLP, vitamin B-12, RBC folate, and homocysteine were identified as predictors of SAA. When stratified by folic acid fortification period, nutritionhomocysteine correlations were generally weaker in the postfortification period, whereas associations between plasma PLP and serum CRP increased. Biomarkers of inflammation are associated with PLP, RBC folate, and homocysteine in women. The connection between the pathways needs to be further investigated and causality established.
UR - http://www.scopus.com/inward/record.url?scp=84898849533&partnerID=8YFLogxK
U2 - 10.3945/jn.113.183970
DO - 10.3945/jn.113.183970
M3 - Article
C2 - 24647390
AN - SCOPUS:84898849533
SN - 0022-3166
VL - 144
SP - 714
EP - 721
JO - Journal of Nutrition
JF - Journal of Nutrition
IS - 5
ER -