TY - JOUR
T1 - Bioluminescent Tumor Signal Is Mouse Strain and Pelt Color Dependent
T2 - Experience in a Disseminated Lymphoma Model
AU - Hoegger, Mark J.
AU - Longtine, Mark S.
AU - Shim, Kyuhwan
AU - Wahl, Richard L.
N1 - Funding Information:
These studies were funded in part by the RSNA Resident Research Grant RR1839. MJH received salary support from National Institutes of Health TOP-TIER grant T32-EB021955 during the study design period.
Publisher Copyright:
© 2021, World Molecular Imaging Society.
PY - 2021/10
Y1 - 2021/10
N2 - Background: Many preclinical cancer studies use mice with varied phenotypes to monitor tumor treatment. We compared survival and optical imaging characteristics of strains with varied coat colors harboring luciferase-expressing disseminated lymphoma. Results: Luciferase-expressing lymphoma cells (Raji-luc) were injected via tail vein into severe combined immunodeficient (SCID) and Rag2-IL2rg (R2G2) mice, and survival was tracked. Tumor signals were obtained by imaging ventral and dorsal aspects of mice. Signal attenuation by isolated mouse pelts was measured in vitro. R2G2 mice had decreased survival compared to SCID mice (17 vs. 32 days, p<0.001) despite similar bioluminescence signal when mice were imaged dorsally (p=0.37). However, signal was 17.3-fold higher in R2G2 mice compared to SCID (p<0.001) when imaged ventrally. Isolated dark R2G2 dorsal pelts attenuated signal more than ventral pelts when placed over cells in vitro. Conclusions: Mouse pelt color and imaging aspect are critical considerations for quantifying bioluminescent tumor signal, and the R2G2 mouse strain may prove useful for preclinical targeted therapy studies.
AB - Background: Many preclinical cancer studies use mice with varied phenotypes to monitor tumor treatment. We compared survival and optical imaging characteristics of strains with varied coat colors harboring luciferase-expressing disseminated lymphoma. Results: Luciferase-expressing lymphoma cells (Raji-luc) were injected via tail vein into severe combined immunodeficient (SCID) and Rag2-IL2rg (R2G2) mice, and survival was tracked. Tumor signals were obtained by imaging ventral and dorsal aspects of mice. Signal attenuation by isolated mouse pelts was measured in vitro. R2G2 mice had decreased survival compared to SCID mice (17 vs. 32 days, p<0.001) despite similar bioluminescence signal when mice were imaged dorsally (p=0.37). However, signal was 17.3-fold higher in R2G2 mice compared to SCID (p<0.001) when imaged ventrally. Isolated dark R2G2 dorsal pelts attenuated signal more than ventral pelts when placed over cells in vitro. Conclusions: Mouse pelt color and imaging aspect are critical considerations for quantifying bioluminescent tumor signal, and the R2G2 mouse strain may prove useful for preclinical targeted therapy studies.
KW - Bioluminescent imaging
KW - Lymphoma
KW - Mice
KW - Preclinical cancer therapy
KW - Targeted radionuclide therapy
UR - http://www.scopus.com/inward/record.url?scp=85104062723&partnerID=8YFLogxK
U2 - 10.1007/s11307-021-01594-0
DO - 10.1007/s11307-021-01594-0
M3 - Article
C2 - 33830414
AN - SCOPUS:85104062723
VL - 23
SP - 697
EP - 702
JO - Molecular Imaging and Biology
JF - Molecular Imaging and Biology
SN - 1536-1632
IS - 5
ER -