Biology of cloned cytotoxic T lymphocytes specific for lymphocyte choriomeningitis virus: Clearance of virus and in vitro properties

J. Anderson, J. A. Byrne, R. Schreiber, S. Patterson, M. B. Oldstone

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

We have generated lymphocytic choriomeningitis virus-specific, H-2-restricted cytotoxic thymus-derived lymphocyte (CTL) clones. By using these reagents in several in vitro assays with infected target cells, we show that CTLs by themselves prevent the release of infectious virus into culture fluids and significantly lower the titers of infectious virus previously produced. This ability of cloned CTLs is not influenced by monensin. However, monenesin does abrogate the ability of CTLs from spleens of mice primed 6 to 8 days previously with virus to kill virus-infected syngeneic targets. When tested for the participation of lymphokines in this system, the CTLs proliferate when reacted with syngeneic lymphocytic choriomeningitis virus-infected macrophages but fail to make interleukin-2. These CTLs make γ interferon when reacted with syngeneic virus-infected targets. However, the production of interferon does not directly correlate with CTL-mediated killing. The number of H-2K and D molecules expressed on the target cell surface is not altered during the course of lymphocytic choriomeningitis virus infection. Electron microscopy shows fingerlike projections of the CTL clone thrust into the infected cell and lesions bearing an internal diameter of approximately 15 nm in those membranes, illustrating the lytic process.

Original languageEnglish
Pages (from-to)552-560
Number of pages9
JournalJournal of virology
Volume53
Issue number2
StatePublished - Apr 4 1985
Externally publishedYes

Fingerprint Dive into the research topics of 'Biology of cloned cytotoxic T lymphocytes specific for lymphocyte choriomeningitis virus: Clearance of virus and in vitro properties'. Together they form a unique fingerprint.

  • Cite this