Biological super-resolution imaging by using novel microsphere-embedded coverslips

Arash Darafsheh; Consuelo Guardiola; Deepak Nihalani; Daeyeon Lee; Jarod C. Finlay; Alejandro Cárabe

doi:10.1117/12.2078019

Biological super-resolution imaging by using novel microsphere-embedded coverslips

Arash Darafsheh, Consuelo Guardiola, Deepak Nihalani, Daeyeon Lee, Jarod C. Finlay, Alejandro Cárabe

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

30 Scopus citations

Abstract

Optical super-resolution imaging is a desirable technique in many fields, including medical and material sciences and nanophotonics. We demonstrated feasibility of optical microscopy, with resolution improvement by a factor of 2-3, by using microsphere-embedded coverslips composed of barium titanate glass microspheres (refractive index n~1.9-2.1) fixed in a transparent layer of polydimethylsiloxane. Imaging was performed by a conventional microscope and a fluorescent microscope with the microsphere-embedded layer placed in a contact position with various biological specimens and semiconductor nanostructures. We investigated a biomedical application of microsphere-assisted imaging technique by immunostaining of kidney sections where cellular distribution of a motor protein Myo1c and a podocyte specific protein ZO-1 was analyzed. A significant visual enhancement in the distribution pattern of the proteins was noted in the stained glomeruli by using microsphere-assisted imaging technique. Our results suggest that microsphere-assisted imaging technique is a promising candidate for applications in medical and cancer research, as well as in microfluidics and nanophotonics.

Original language	English
Title of host publication	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII
Editors	Dan V. Nicolau, Alexander N. Cartwright
Publisher	SPIE
ISBN (Electronic)	9781628414271
DOIs	https://doi.org/10.1117/12.2078019
State	Published - 2015
Event	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII - San Francisco, United States Duration: Feb 9 2015 → Feb 12 2015

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9337
ISSN (Print)	1605-7422

Conference

Conference	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII
Country/Territory	United States
City	San Francisco
Period	02/9/15 → 02/12/15

Keywords

PDMS
Super-resolution
barium titanate
biological imaging
microscopy
microsphere
nanoscopy

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10.1117/12.2078019

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Darafsheh, A., Guardiola, C., Nihalani, D., Lee, D., Finlay, J. C., & Cárabe, A. (2015). Biological super-resolution imaging by using novel microsphere-embedded coverslips. In D. V. Nicolau, & A. N. Cartwright (Eds.), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII Article 933705 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9337). SPIE. https://doi.org/10.1117/12.2078019

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title = "Biological super-resolution imaging by using novel microsphere-embedded coverslips",

abstract = "Optical super-resolution imaging is a desirable technique in many fields, including medical and material sciences and nanophotonics. We demonstrated feasibility of optical microscopy, with resolution improvement by a factor of 2-3, by using microsphere-embedded coverslips composed of barium titanate glass microspheres (refractive index n~1.9-2.1) fixed in a transparent layer of polydimethylsiloxane. Imaging was performed by a conventional microscope and a fluorescent microscope with the microsphere-embedded layer placed in a contact position with various biological specimens and semiconductor nanostructures. We investigated a biomedical application of microsphere-assisted imaging technique by immunostaining of kidney sections where cellular distribution of a motor protein Myo1c and a podocyte specific protein ZO-1 was analyzed. A significant visual enhancement in the distribution pattern of the proteins was noted in the stained glomeruli by using microsphere-assisted imaging technique. Our results suggest that microsphere-assisted imaging technique is a promising candidate for applications in medical and cancer research, as well as in microfluidics and nanophotonics.",

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author = "Arash Darafsheh and Consuelo Guardiola and Deepak Nihalani and Daeyeon Lee and Finlay, {Jarod C.} and Alejandro C{\'a}rabe",

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Darafsheh, A, Guardiola, C, Nihalani, D, Lee, D, Finlay, JC & Cárabe, A 2015, Biological super-resolution imaging by using novel microsphere-embedded coverslips. in DV Nicolau & AN Cartwright (eds), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII., 933705, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9337, SPIE, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII, San Francisco, United States, 02/9/15. https://doi.org/10.1117/12.2078019

Biological super-resolution imaging by using novel microsphere-embedded coverslips. / Darafsheh, Arash; Guardiola, Consuelo; Nihalani, Deepak et al.
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII. ed. / Dan V. Nicolau; Alexander N. Cartwright. SPIE, 2015. 933705 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9337).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Cárabe, Alejandro

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AB - Optical super-resolution imaging is a desirable technique in many fields, including medical and material sciences and nanophotonics. We demonstrated feasibility of optical microscopy, with resolution improvement by a factor of 2-3, by using microsphere-embedded coverslips composed of barium titanate glass microspheres (refractive index n~1.9-2.1) fixed in a transparent layer of polydimethylsiloxane. Imaging was performed by a conventional microscope and a fluorescent microscope with the microsphere-embedded layer placed in a contact position with various biological specimens and semiconductor nanostructures. We investigated a biomedical application of microsphere-assisted imaging technique by immunostaining of kidney sections where cellular distribution of a motor protein Myo1c and a podocyte specific protein ZO-1 was analyzed. A significant visual enhancement in the distribution pattern of the proteins was noted in the stained glomeruli by using microsphere-assisted imaging technique. Our results suggest that microsphere-assisted imaging technique is a promising candidate for applications in medical and cancer research, as well as in microfluidics and nanophotonics.

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Darafsheh A, Guardiola C, Nihalani D, Lee D, Finlay JC, Cárabe A. Biological super-resolution imaging by using novel microsphere-embedded coverslips. In Nicolau DV, Cartwright AN, editors, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII. SPIE. 2015. 933705. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2078019

Biological super-resolution imaging by using novel microsphere-embedded coverslips

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