Biolistic transfection

Paul C. Bridgman; Michael E. Brown; Irina Balan

doi:10.1016/s0091-679x(03)01017-3

Biolistic transfection

Paul C. Bridgman, Michael E. Brown, Irina Balan

Research output: Contribution to journal › Review article › peer-review

12 Scopus citations

Abstract

Recent improvements in the biolistic technique and devices have increased its usefulness for transfection of neurons. With these recent advances, both dissociated and slice cultures can be transfected at reasonably high rates. This chapter focuses on the parameters that determine the successful biolistic transfection of neurons in both types of cultures.

Original language	English
Pages (from-to)	353-368
Number of pages	16
Journal	Methods in cell biology
Volume	2003
Issue number	71
DOIs	https://doi.org/10.1016/s0091-679x(03)01017-3
State	Published - 2003

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@article{a0823af469f6458687507d20fcbfd563,

title = "Biolistic transfection",

abstract = "Recent improvements in the biolistic technique and devices have increased its usefulness for transfection of neurons. With these recent advances, both dissociated and slice cultures can be transfected at reasonably high rates. This chapter focuses on the parameters that determine the successful biolistic transfection of neurons in both types of cultures.",

author = "Bridgman, {Paul C.} and Brown, {Michael E.} and Irina Balan",

note = "Funding Information: This work was supported by NIH Grants NS26150 and NS35162 to P.C.B. Dr. Michael Nonet developed the original prototype for the custom gene gun that we describe.",

year = "2003",

doi = "10.1016/s0091-679x(03)01017-3",

language = "English",

volume = "2003",

pages = "353--368",

journal = "Methods in cell biology",

issn = "0091-679X",

number = "71",

}

TY - JOUR

T1 - Biolistic transfection

AU - Bridgman, Paul C.

AU - Brown, Michael E.

AU - Balan, Irina

N1 - Funding Information: This work was supported by NIH Grants NS26150 and NS35162 to P.C.B. Dr. Michael Nonet developed the original prototype for the custom gene gun that we describe.

PY - 2003

Y1 - 2003

N2 - Recent improvements in the biolistic technique and devices have increased its usefulness for transfection of neurons. With these recent advances, both dissociated and slice cultures can be transfected at reasonably high rates. This chapter focuses on the parameters that determine the successful biolistic transfection of neurons in both types of cultures.

AB - Recent improvements in the biolistic technique and devices have increased its usefulness for transfection of neurons. With these recent advances, both dissociated and slice cultures can be transfected at reasonably high rates. This chapter focuses on the parameters that determine the successful biolistic transfection of neurons in both types of cultures.

UR - http://www.scopus.com/inward/record.url?scp=1542779787&partnerID=8YFLogxK

U2 - 10.1016/s0091-679x(03)01017-3

DO - 10.1016/s0091-679x(03)01017-3

M3 - Review article

C2 - 12884699

AN - SCOPUS:1542779787

SN - 0091-679X

VL - 2003

SP - 353

EP - 368

JO - Methods in cell biology

JF - Methods in cell biology

IS - 71

ER -

Biolistic transfection

Abstract

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