TY - JOUR
T1 - Bioinformatics analysis of small RNAs in plants using next generation sequencing technologies.
AU - Nobuta, Kan
AU - McCormick, Kevin
AU - Nakano, Mayumi
AU - Meyers, Blake C.
PY - 2010
Y1 - 2010
N2 - Next-generation sequencing technologies have a substantial impact on a broad range of biological applications. Like many other groups, we use these new technologies, especially SBS (Sequence-By-Synthesis), for deep profiling of small RNA molecules in plants. Small RNAs are 21-24 nucleotides in length and are known to play a major role in the activation of mRNAs and genomic DNAs. We have generated numerous SBS small RNA libraries; each can consist of more than three million signatures of more than 33 nucleotides in length. Here, we describe the challenges and our strategies to handle the very large quantity of small RNA data generated by these next-generation sequencing technologies.
AB - Next-generation sequencing technologies have a substantial impact on a broad range of biological applications. Like many other groups, we use these new technologies, especially SBS (Sequence-By-Synthesis), for deep profiling of small RNA molecules in plants. Small RNAs are 21-24 nucleotides in length and are known to play a major role in the activation of mRNAs and genomic DNAs. We have generated numerous SBS small RNA libraries; each can consist of more than three million signatures of more than 33 nucleotides in length. Here, we describe the challenges and our strategies to handle the very large quantity of small RNA data generated by these next-generation sequencing technologies.
UR - http://www.scopus.com/inward/record.url?scp=74049155078&partnerID=8YFLogxK
U2 - 10.1007/978-1-60327-005-2_7
DO - 10.1007/978-1-60327-005-2_7
M3 - Article
C2 - 19802591
AN - SCOPUS:74049155078
SN - 1064-3745
VL - 592
SP - 89
EP - 106
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -