Binding of poly (Glu60Ala30Tyr10) by thymic lymphocytes from genetic responder and non responder mice: effect of antihistocompatibility serum

L. J. Kennedy, M. E. Dorf, E. R. Unanue, B. B. Cerraf

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The binding of 125I labeled GAT by murine thymocytes was studied by autoradiography. GAT binding was highly temperature dependent. Significantly more thymocytes bound GAT at 37°C than at 4°C. GAT bound at 37°C was usually distributed in a 'cap' over one pole of the cell. Preincubation of thymocytes with specific anti H 2 serum markedly inhibited the subsequent binding of GAT. The frequency of antigen binding thymocytes was not significantly affected by preincubation of thymocytes with anti Thy 1 serum or a number of anti immunoglobulin in sera. The immune response to GAT is controlled by an H 2 linked immune response gene(s). Mice of strains that are H 2(p), H 2(q) or H 2(s) (non responders) fail to develop effective T cell helper function necessary for a humoral immune response to GAT under most conditions. However, both responder and non responder strains had similar numbers of thymocytes capable of binding GAT in vitro, indicating that T cells from non responder strains of mice do possess a recognition mechanism for GAT.

Original languageEnglish
Pages (from-to)1670-1675
Number of pages6
JournalJournal of Immunology
Volume114
Issue number6
StatePublished - Dec 1 1975
Externally publishedYes

Fingerprint Dive into the research topics of 'Binding of poly (Glu<sup>60</sup>Ala<sup>30</sup>Tyr<sup>10</sup>) by thymic lymphocytes from genetic responder and non responder mice: effect of antihistocompatibility serum'. Together they form a unique fingerprint.

  • Cite this