Beta-cell injury in Ncb5or-null mice is exacerbated by consumption of a high-fat diet

Ying Guo, Ming Xu, Bin Deng, Jennifer R. Frontera, Karen L. Kover, Daniel Aires, Helin Ding, Susan E. Carlson, John Turk, Wenfang Wang, Hao Zhu

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


NADH-cytochrome b5 oxidoreductase (Ncb5or) in endoplasmic reticulum (ER) is involved in fatty acid metabolism, and Ncb5or -/- mice fed standard chow (SC) are insulin-sensitive but weigh less than wild-type (WT) littermates. Ncb5or -/- mice develop hyperglycemia at about age 7 wk due to β-cell dysfunction and loss in association with saturated fatty acid (SFA) accumulation and manifestations of ER and oxidative stress. Here we report that when Ncb5or -/- mice born to heterozygous mothers fed a high fat (HF) diet continue to ingest HF, they weigh as much as SC-fed WT at age 5 wk. By age 7 wk, diabetes mellitus develops in all HF-fed versus 68% of SC-fed Ncb5or -/- mice. Islet β-cell content in age 5-wk Ncb5or -/- mice fed HF for 7 days is lower (53%) than for those fed SC (63%), and both are lower than for WT (75%, SC, vs. 69%, HF). Islet transcript levels for markers of mitochondrial biogenesis (PGC-1α) and ER stress (ATF6α) are higher in Ncb5or -/- than WT mice but not significantly affected by diet. Consuming a HF diet exacerbates Ncb5or -/- β-cell accumulation of intracellular SFA and increases the frequency of ER distention from 11% (SC) to 47% (HF), thus accelerates β-cell injury in Ncb5or -/- mice.

Original languageEnglish
Pages (from-to)233-243
Number of pages11
JournalEuropean Journal of Lipid Science and Technology
Issue number3
StatePublished - Mar 2012


  • Beta-cells
  • Diabetes
  • ER stress
  • High-fat diet
  • Lipotoxicity


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