TY - JOUR
T1 - Behavioral and physiological effects of acute ketamine exposure in adult zebrafish
AU - Riehl, Russell
AU - Kyzar, Evan
AU - Allain, Alexander
AU - Green, Jeremy
AU - Hook, Molly
AU - Monnig, Louis
AU - Rhymes, Kate
AU - Roth, Andrew
AU - Pham, Mimi
AU - Razavi, Roshan
AU - DiLeo, John
AU - Gaikwad, Siddharth
AU - Hart, Peter
AU - Kalueff, Allan V.
N1 - Funding Information:
The study was supported by the Zebrafish Neuroscience Research Consortium (ZNRC) , Tulane Neurophenotyping Platform and the Tulane School of Medicine Pilot Program grants. Funders had no involvement in the study design, data collection and analysis, or the preparation of this MS.
PY - 2011/11
Y1 - 2011/11
N2 - Ketamine is a non-competitive glutamatergic antagonist used to induce sedation and analgesia. In sub-anesthetic doses, it induces hyperlocomotion, impairs memory and evokes stereotypic circling in rodents. Zebrafish (Danio rerio) emerged as a promising new animal model to screen the effects of psychotropic compounds. Here, we investigated the effects of sub-anesthetic doses of ketamine on anxiety, locomotion, habituation and social behavior of adult zebrafish. Acute 20-min exposure to 20 and 40. mg/L (but not 2. mg/L) of ketamine reduced anxiety, impaired intra-session habituation, evoked circular swimming and disrupted zebrafish shoaling. Additionally, ketamine reduced whole-body cortisol levels and elevated brain c-fos expression in zebrafish. Our findings demonstrate the sensitivity of zebrafish to behavioral and physiological effects of sub-anesthetic doses of ketamine, further supporting the utility of this species as a model for neuropharmacological research, including testing ketamine and related drugs.
AB - Ketamine is a non-competitive glutamatergic antagonist used to induce sedation and analgesia. In sub-anesthetic doses, it induces hyperlocomotion, impairs memory and evokes stereotypic circling in rodents. Zebrafish (Danio rerio) emerged as a promising new animal model to screen the effects of psychotropic compounds. Here, we investigated the effects of sub-anesthetic doses of ketamine on anxiety, locomotion, habituation and social behavior of adult zebrafish. Acute 20-min exposure to 20 and 40. mg/L (but not 2. mg/L) of ketamine reduced anxiety, impaired intra-session habituation, evoked circular swimming and disrupted zebrafish shoaling. Additionally, ketamine reduced whole-body cortisol levels and elevated brain c-fos expression in zebrafish. Our findings demonstrate the sensitivity of zebrafish to behavioral and physiological effects of sub-anesthetic doses of ketamine, further supporting the utility of this species as a model for neuropharmacological research, including testing ketamine and related drugs.
KW - Anxiety
KW - Aquatic models
KW - Behavioral testing
KW - C-fos expression
KW - Cortisol
KW - Locomotion and circling
UR - http://www.scopus.com/inward/record.url?scp=84855955477&partnerID=8YFLogxK
U2 - 10.1016/j.ntt.2011.05.011
DO - 10.1016/j.ntt.2011.05.011
M3 - Article
C2 - 21683787
AN - SCOPUS:84855955477
SN - 0892-0362
VL - 33
SP - 658
EP - 667
JO - Neurotoxicology and Teratology
JF - Neurotoxicology and Teratology
IS - 6
ER -