Bcl11a Controls Flt3 Expression in Early Hematopoietic Progenitors and Is Required for pDC Development In Vivo

Xiaodi Wu, Ansuman T. Satpathy, Wumesh Kc, Pentao Liu, Theresa L. Murphy, Kenneth M. Murphy

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Bcl11a is a transcription factor known to regulate lymphoid and erythroid development. Recent bioinformatic analysis of global gene expression patterns has suggested a role for Bcl11a in the development of dendritic cell (DC) lineages. We tested this hypothesis by analyzing the development of DC and other lineages in Bcl11a-/- mice. We found that Bcl11a was required for expression of IL-7 receptor (IL-7R) and Flt3 in early hematopoietic progenitor cells. In addition, we found severely decreased numbers of plasmacytoid dendritic cells (pDCs) in Bcl11a-/- fetal livers and in the bone marrow of Bcl11a-/- fetal liver chimeras. Moreover, Bcl11a-/- cells showed severely impaired in vitro development of Flt3L-derived pDCs and classical DCs (cDCs). In contrast, we found normal in vitro development of DCs from Bcl11a-/- fetal liver cells treated with GM-CSF. These results suggest that the persistent cDC development observed in Bcl11a-/- fetal liver chimeras reflects derivation from a Bcl11a- and Flt3-independent pathway in vivo.

Original languageEnglish
Article numbere64800
JournalPloS one
Volume8
Issue number5
DOIs
StatePublished - May 31 2013

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