Bcl-2/JH gene re-arrangement determined by PCR as a means to detect minimal residual disease in malignant lymphomas

X. Zhifu, C. Yan, L. Chongyu

Research output: Contribution to journalArticle

Abstract

Objective: To develop a sensitive method to detect minimal residual disease and to elucidate the significance of bcl-2 gene rearrangement in diagnosis and therapy of malignant lymphoma. Methods: Using polymerase chain reaction(PCR) to detect bcl-2 gene rearrangement and using serial dilution method to define the sensitivity of PCR. Results: In 9 different malignant lymphoma cell lines, Su-DHL-4 and Su-DHL-6 were shown bcl-2(MBR)/JH rearrangement, the sensitivity of PCR was 1: 105. In 16 patients with follicular lymphoma, the peripheral blood and bone marrow were PCR positive in 4 both at initial diagnosis and after complete remission. Conclusion: Detection of bcl-2 gene rearrangement by PCR provides a sensitive and specific assay of minimal residual disease. It helps improve staging of disease, stratification and evaluation of treatment.

Original languageEnglish
Pages (from-to)290-292
Number of pages3
JournalChinese Journal of Oncology
Volume20
Issue number4
StatePublished - Sep 28 1998
Externally publishedYes

Keywords

  • Bcl-2 gene
  • Gene rearrangement
  • Lymphoma
  • Polymerase chain reaction

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