bcl-2 gene rearrangement determined by PCR as a mean to detect minimal residual disease in malignant lymphomas

Z. F. Xiang, Y. Y. Lu, Y. R. Lai, Y. Chen, H. Y. Li, P. Zou

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Abstract

Objective: To develop a sensitive method to detect minimal residual disease and to elucidate the significance of bcl-2 gene rearrangement in diagnosis and treatment of malignant lymphoma. Methods: Using polymerase chain reaction (PCR) to detect bcl-2 gene rearrangement and using serial dilution method to define the sensitivity of PCR. Results: In 9 different malignant lymphoma cell lines, Su-DHL-4 and Su-DHL6 were shown bcl-2(MBR)/JH rearrangement, the sensitivity of PCR was 1:105. In 16 patients with follicular lymphoma, the peripheral blood and bone marrow were PCR positive in 4 cases both at initial diagnosis and after complete remission. Conclusion: Detection of bcl-2 gene rearrangement by PCR provides a sensitive and specific assay of minimal residual disease. It is helpful to improve staging of disease, prognosis and evaluation of the treatment results.

Original languageEnglish
Pages (from-to)49-52
Number of pages4
JournalChinese Journal of Cancer Research
Volume11
Issue number1
DOIs
StatePublished - 1999

Keywords

  • Gene rearrangement
  • Lymphoma
  • Polymerase chain reaction
  • bcl-2 gene

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