TY - JOUR
T1 - Battery-free, lightweight, injectable microsystem for in vivo wireless pharmacology and optogenetics
AU - Zhang, Yi
AU - Castro, Daniel C.
AU - Han, Yuan
AU - Wu, Yixin
AU - Guo, Hexia
AU - Weng, Zhengyan
AU - Xue, Yeguang
AU - Ausra, Jokubas
AU - Wang, Xueju
AU - Li, Rui
AU - Wu, Guangfu
AU - Vázquez-Guardado, Abraham
AU - Xie, Yiwen
AU - Xie, Zhaoqian
AU - Ostojich, Diana
AU - Peng, Dongsheng
AU - Sun, Rujie
AU - Wang, Binbin
AU - Yu, Yongjoon
AU - Leshock, John P.
AU - Qu, Subing
AU - Su, Chun Ju
AU - Shen, Wen
AU - Hang, Tao
AU - Banks, Anthony
AU - Huang, Yonggang
AU - Radulovic, Jelena
AU - Gutruf, Philipp
AU - Bruchas, Michael R.
AU - Rogers, John A.
N1 - Funding Information:
This work was supported by NIH Brain Research through Advancing Innovative Neurotechnologies (BRAIN) Initiative Grant R21EY027612A (to M.R.B. and J.A.R.), National Institute of Mental Health (NIMH) Grants R01MH112355 (to M.R.B.) and R41MH116525 (to NeuroLux, Inc.), NIMH Grants MH108837 and MH078064 (to J.R.), National Institute on Drug Abuse (NIDA) Grant DA044121 (to J.R.), NIDA F32 Fellowship DA043999-02 (to D.C.C.), National Natural Science Foundation of China Fellowship 81571069 (to Y. Han), University of Arizona Start-up fund (P.G.), and the University of Missouri?Columbia start?up fund (Y.Z.). R.L. acknowledges support from LiaoNing Revitalization Talents Program (Grant XLYC1807126), Young Elite Scientists Sponsorship Program by China Association for Science and Technology (CAST) (Grant 2015QNRC001), and Fundamental Research Funds for the Central Universities of China (Grant DUT18GF101).
Funding Information:
ACKNOWLEDGMENTS. This work was supported by NIH Brain Research through Advancing Innovative Neurotechnologies (BRAIN) Initiative Grant R21EY027612A (to M.R.B. and J.A.R.), National Institute of Mental Health (NIMH) Grants R01MH112355 (to M.R.B.) and R41MH116525 (to NeuroLux, Inc.), NIMH Grants MH108837 and MH078064 (to J.R.), National Institute on Drug Abuse (NIDA) Grant DA044121 (to J.R.), NIDA F32 Fellowship DA043999-02 (to D.C.C.), National Natural Science Foundation of China Fellowship 81571069 (to Y. Han), University of Arizona Start-up fund (P.G.), and the University of Missouri‐Columbia start‐up fund (Y.Z.). R.L. acknowledges support from LiaoNing Revitalization Talents Program (Grant XLYC1807126), Young Elite Scientists Sponsorship Program by China Association for Science and Technology (CAST) (Grant 2015QNRC001), and Fundamental Research Funds for the Central Universities of China (Grant DUT18GF101).
Publisher Copyright:
© 2019 National Academy of Sciences. All rights reserved.
PY - 2019/10/22
Y1 - 2019/10/22
N2 - Pharmacology and optogenetics are widely used in neuroscience research to study the central and peripheral nervous systems. While both approaches allow for sophisticated studies of neural circuitry, continued advances are, in part, hampered by technology limitations associated with requirements for physical tethers that connect external equipment to rigid probes inserted into delicate regions of the brain. The results can lead to tissue damage and alterations in behavioral tasks and natural movements, with additional difficulties in use for studies that involve social interactions and/or motions in complex 3-dimensional environments. These disadvantages are particularly pronounced in research that demands combined optogenetic and pharmacological functions in a single experiment. Here, we present a lightweight, wireless, battery-free injectable microsystem that combines soft microfluidic and microscale inorganic light-emitting diode probes for programmable pharmacology and optogenetics, designed to offer the features of drug refillability and adjustable flow rates, together with programmable control over the temporal profiles. The technology has potential for large-scale manufacturing and broad distribution to the neuroscience community, with capabilities in targeting specific neuronal populations in freely moving animals. In addition, the same platform can easily be adapted for a wide range of other types of passive or active electronic functions, including electrical stimulation.
AB - Pharmacology and optogenetics are widely used in neuroscience research to study the central and peripheral nervous systems. While both approaches allow for sophisticated studies of neural circuitry, continued advances are, in part, hampered by technology limitations associated with requirements for physical tethers that connect external equipment to rigid probes inserted into delicate regions of the brain. The results can lead to tissue damage and alterations in behavioral tasks and natural movements, with additional difficulties in use for studies that involve social interactions and/or motions in complex 3-dimensional environments. These disadvantages are particularly pronounced in research that demands combined optogenetic and pharmacological functions in a single experiment. Here, we present a lightweight, wireless, battery-free injectable microsystem that combines soft microfluidic and microscale inorganic light-emitting diode probes for programmable pharmacology and optogenetics, designed to offer the features of drug refillability and adjustable flow rates, together with programmable control over the temporal profiles. The technology has potential for large-scale manufacturing and broad distribution to the neuroscience community, with capabilities in targeting specific neuronal populations in freely moving animals. In addition, the same platform can easily be adapted for a wide range of other types of passive or active electronic functions, including electrical stimulation.
KW - Neuroscience
KW - Optogenetics
KW - Pharmacology
UR - http://www.scopus.com/inward/record.url?scp=85073759453&partnerID=8YFLogxK
U2 - 10.1073/pnas.1909850116
DO - 10.1073/pnas.1909850116
M3 - Article
C2 - 31601737
AN - SCOPUS:85073759453
VL - 116
SP - 21427
EP - 21437
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 43
ER -