TY - JOUR
T1 - Batf3 deficiency reveals a critical role for CD8α+ dendritic cells in cytotoxic T cell immunity
AU - Hildner, Kai
AU - Edelson, Brian T.
AU - Purtha, Whitney E.
AU - Diamond, Mark
AU - Matsushita, Hirokazu
AU - Kohyama, Masako
AU - Calderon, Boris
AU - Schraml, Barbara U.
AU - Unanue, Emil R.
AU - Diamond, Michael S.
AU - Schreiber, Robert D.
AU - Murphy, Theresa L.
AU - Murphy, Kenneth M.
PY - 2008/11/14
Y1 - 2008/11/14
N2 - Although in vitro observations suggest that cross-presentation of antigens is mediated primarily by CD8α+ dendritic cells, in vivo analysis has been hampered by the lack of systems that selectively eliminate this cell lineage. We show that deletion of the transcription factor Batf3 ablated development of CD8α+ dendritic cells, allowing us to examine their role in immunity in vivo. Dendritic cells from Batf3-/- mice were defective in cross-presentation, and Batf3-/- mice lacked virus-specific CD8+ T cell responses to West Nile virus. Importantly, rejection of highly immunogenic syngeneic tumors was impaired in Batf3 -/- mice. These results suggest an important role for CD8α+ dendritic cells and cross-presentation in responses to viruses and in tumor rejection.
AB - Although in vitro observations suggest that cross-presentation of antigens is mediated primarily by CD8α+ dendritic cells, in vivo analysis has been hampered by the lack of systems that selectively eliminate this cell lineage. We show that deletion of the transcription factor Batf3 ablated development of CD8α+ dendritic cells, allowing us to examine their role in immunity in vivo. Dendritic cells from Batf3-/- mice were defective in cross-presentation, and Batf3-/- mice lacked virus-specific CD8+ T cell responses to West Nile virus. Importantly, rejection of highly immunogenic syngeneic tumors was impaired in Batf3 -/- mice. These results suggest an important role for CD8α+ dendritic cells and cross-presentation in responses to viruses and in tumor rejection.
UR - http://www.scopus.com/inward/record.url?scp=56449097442&partnerID=8YFLogxK
U2 - 10.1126/science.1164206
DO - 10.1126/science.1164206
M3 - Article
C2 - 19008445
AN - SCOPUS:56449097442
VL - 322
SP - 1097
EP - 1100
JO - Science
JF - Science
SN - 0036-8075
IS - 5904
ER -