Basement membrane ligands initiate distinct signalling networks to direct cell shape

Michael J. Randles, Franziska Lausecker, Jonathan D. Humphries, Adam Byron, Simon J. Clark, Jeffrey H. Miner, Roy Zent, Martin J. Humphries, Rachel Lennon

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Cells have evolved mechanisms to sense the composition of their adhesive microenvironment. Although much is known about general mechanisms employed by adhesion receptors to relay signals between the extracellular environment and the cytoskeleton, the nuances of ligand-specific signalling remain undefined. Here, we investigated how glomerular podocytes, and four other basement membrane-associated cell types, respond morphologically to different basement membrane ligands. We defined the composition of the respective adhesion complexes using mass spectrometry-based proteomics. On type IV collagen, all epithelial cell types adopted a round morphology, with a single lamellipodium and large adhesion complexes rich in actin-binding proteins. On laminin (511 or 521), all cell types attached to a similar degree but were polygonal in shape with small adhesion complexes enriched in endocytic and microtubule-binding proteins. Consistent with their distinctive morphologies, cells on type IV collagen exhibited high Rac1 activity, while those on laminin had elevated PKCα. Perturbation of PKCα was able to interchange morphology consistent with a key role for this pathway in matrix ligand-specific signalling. Therefore, this study defines the switchable basement membrane adhesome and highlights two key signalling pathways within the systems that determine distinct cell morphologies. Proteomic data are available via ProteomeXchange with identifier PXD017913.

Original languageEnglish
Pages (from-to)61-78
Number of pages18
JournalMatrix Biology
Volume90
DOIs
StatePublished - Aug 2020

Keywords

  • Adhesome
  • Basement membrane
  • Cell shape
  • Laminin
  • PKC
  • Rac1
  • Type IV collagen

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