Barbiturate and picrotoxin-sensitive chloride efflux in rat cerebral cortical synaptoneurosomes

Rochelle D. Schwartz; Phil Skolnick; Elizabeth B. Hollingsworth; Steven M. Paul

doi:10.1016/0014-5793(84)80597-9

Barbiturate and picrotoxin-sensitive chloride efflux in rat cerebral cortical synaptoneurosomes

Rochelle D. Schwartz, Phil Skolnick, Elizabeth B. Hollingsworth, Steven M. Paul

Washington University School of Medicine

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

The effects of various barbiturates and picrotoxin in modifying the efflux of chloride (³⁶Cl^-) was studied in a novel subcellular preparation from rat cerebral cortex, the 'synaptoneurosome'. Dilution of synaptoneurosomes pre-loaded with ³⁶Cl^- resulted in rapid efflux of ³⁶Cl^- that could be measured as early as 10 s following dilution. In the presence of barbiturates such as pentobarbital and hexobarbital there was a significant increase in ³⁶Cl^- efflux which was not observed with the pharmacologically-inactive barbiturate, barbital. The effect of barbiturates in enhancing ³⁶Cl^- efflux was also stereospecific [(-)-DMBB > (+)-DMBB] and reversed by picrotoxin. By contrast, picrotoxin alone significantly inhibited ³⁶Cl^- efflux. These data demonstrate pharmacologically relevant Cl^- transport for the first time in a subcellular brain preparation.

Original language	English
Pages (from-to)	193-196
Number of pages	4
Journal	FEBS Letters
Volume	175
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(84)80597-9
State	Published - Sep 17 1984

Keywords

Barbiturate
Brain synaptoneurosome
Chloride efflux
GABA
Picrotoxin

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10.1016/0014-5793(84)80597-9

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title = "Barbiturate and picrotoxin-sensitive chloride efflux in rat cerebral cortical synaptoneurosomes",

abstract = "The effects of various barbiturates and picrotoxin in modifying the efflux of chloride (36Cl-) was studied in a novel subcellular preparation from rat cerebral cortex, the 'synaptoneurosome'. Dilution of synaptoneurosomes pre-loaded with 36Cl- resulted in rapid efflux of 36Cl- that could be measured as early as 10 s following dilution. In the presence of barbiturates such as pentobarbital and hexobarbital there was a significant increase in 36Cl- efflux which was not observed with the pharmacologically-inactive barbiturate, barbital. The effect of barbiturates in enhancing 36Cl- efflux was also stereospecific [(-)-DMBB > (+)-DMBB] and reversed by picrotoxin. By contrast, picrotoxin alone significantly inhibited 36Cl- efflux. These data demonstrate pharmacologically relevant Cl- transport for the first time in a subcellular brain preparation.",

keywords = "Barbiturate, Brain synaptoneurosome, Chloride efflux, GABA, Picrotoxin",

author = "Schwartz, {Rochelle D.} and Phil Skolnick and Hollingsworth, {Elizabeth B.} and Paul, {Steven M.}",

year = "1984",

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T1 - Barbiturate and picrotoxin-sensitive chloride efflux in rat cerebral cortical synaptoneurosomes

AU - Schwartz, Rochelle D.

AU - Skolnick, Phil

AU - Hollingsworth, Elizabeth B.

AU - Paul, Steven M.

PY - 1984/9/17

Y1 - 1984/9/17

N2 - The effects of various barbiturates and picrotoxin in modifying the efflux of chloride (36Cl-) was studied in a novel subcellular preparation from rat cerebral cortex, the 'synaptoneurosome'. Dilution of synaptoneurosomes pre-loaded with 36Cl- resulted in rapid efflux of 36Cl- that could be measured as early as 10 s following dilution. In the presence of barbiturates such as pentobarbital and hexobarbital there was a significant increase in 36Cl- efflux which was not observed with the pharmacologically-inactive barbiturate, barbital. The effect of barbiturates in enhancing 36Cl- efflux was also stereospecific [(-)-DMBB > (+)-DMBB] and reversed by picrotoxin. By contrast, picrotoxin alone significantly inhibited 36Cl- efflux. These data demonstrate pharmacologically relevant Cl- transport for the first time in a subcellular brain preparation.

AB - The effects of various barbiturates and picrotoxin in modifying the efflux of chloride (36Cl-) was studied in a novel subcellular preparation from rat cerebral cortex, the 'synaptoneurosome'. Dilution of synaptoneurosomes pre-loaded with 36Cl- resulted in rapid efflux of 36Cl- that could be measured as early as 10 s following dilution. In the presence of barbiturates such as pentobarbital and hexobarbital there was a significant increase in 36Cl- efflux which was not observed with the pharmacologically-inactive barbiturate, barbital. The effect of barbiturates in enhancing 36Cl- efflux was also stereospecific [(-)-DMBB > (+)-DMBB] and reversed by picrotoxin. By contrast, picrotoxin alone significantly inhibited 36Cl- efflux. These data demonstrate pharmacologically relevant Cl- transport for the first time in a subcellular brain preparation.

KW - Barbiturate

KW - Brain synaptoneurosome

KW - Chloride efflux

KW - GABA

KW - Picrotoxin

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ER -

Barbiturate and picrotoxin-sensitive chloride efflux in rat cerebral cortical synaptoneurosomes

Abstract

Keywords

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