TY - JOUR
T1 - Banff Histopathological Consensus Criteria for Preimplantation Kidney Biopsies
AU - the Banff Working Group
AU - Liapis, H.
AU - Gaut, J. P.
AU - Klein, C.
AU - Bagnasco, S.
AU - Kraus, E.
AU - Farris, A. B.
AU - Honsova, E.
AU - Perkowska-Ptasinska, A.
AU - David, D.
AU - Goldberg, J.
AU - Smith, M.
AU - Mengel, M.
AU - Haas, M.
AU - Seshan, S.
AU - Pegas, K. L.
AU - Horwedel, T.
AU - Paliwa, Y.
AU - Gao, X.
AU - Landsittel, D.
AU - Randhawa, P.
N1 - Publisher Copyright:
© Copyright 2016 The American Society of Transplantation and the American Society of Transplant Surgeons
PY - 2017/1/1
Y1 - 2017/1/1
N2 - The Banff working group on preimplantation biopsy was established to develop consensus criteria (best practice guidelines) for the interpretation of preimplantation kidney biopsies. Digitally scanned slides were used (i) to evaluate interobserver variability of histopathologic findings, comparing frozen sections with formalin-fixed, paraffin-embedded tissue of wedge and needle core biopsies, and (ii) to correlate consensus histopathologic findings with graft outcome in a cohort of biopsies from international medical centers. Intraclass correlations (ICCs) and univariable and multivariable statistical analyses were performed. Good to fair reproducibility was observed in semiquantitative scores for percentage of glomerulosclerosis, arterial intimal fibrosis and interstitial fibrosis on frozen wedge biopsies. Evaluation of frozen wedge and core biopsies was comparable for number of glomeruli, but needle biopsies showed worse ICCs for glomerulosclerosis, interstitial fibrosis and tubular atrophy. A consensus evaluation form is provided to help standardize the reporting of histopathologic lesions in donor biopsies. It should be recognized that histologic parameters may not correlate with graft outcome in studies based on organs deemed to be acceptable after careful clinical assessment. Significant limitations remain in the assessment of implantation biopsies.
AB - The Banff working group on preimplantation biopsy was established to develop consensus criteria (best practice guidelines) for the interpretation of preimplantation kidney biopsies. Digitally scanned slides were used (i) to evaluate interobserver variability of histopathologic findings, comparing frozen sections with formalin-fixed, paraffin-embedded tissue of wedge and needle core biopsies, and (ii) to correlate consensus histopathologic findings with graft outcome in a cohort of biopsies from international medical centers. Intraclass correlations (ICCs) and univariable and multivariable statistical analyses were performed. Good to fair reproducibility was observed in semiquantitative scores for percentage of glomerulosclerosis, arterial intimal fibrosis and interstitial fibrosis on frozen wedge biopsies. Evaluation of frozen wedge and core biopsies was comparable for number of glomeruli, but needle biopsies showed worse ICCs for glomerulosclerosis, interstitial fibrosis and tubular atrophy. A consensus evaluation form is provided to help standardize the reporting of histopathologic lesions in donor biopsies. It should be recognized that histologic parameters may not correlate with graft outcome in studies based on organs deemed to be acceptable after careful clinical assessment. Significant limitations remain in the assessment of implantation biopsies.
KW - biopsy
KW - clinical research/practice
KW - donors and donation: deceased
KW - kidney (allograft) function/dysfunction
KW - kidney failure/injury
KW - pathology/histopathology
UR - http://www.scopus.com/inward/record.url?scp=84995685158&partnerID=8YFLogxK
U2 - 10.1111/ajt.13929
DO - 10.1111/ajt.13929
M3 - Article
C2 - 27333454
AN - SCOPUS:84995685158
SN - 1600-6135
VL - 17
SP - 140
EP - 150
JO - American Journal of Transplantation
JF - American Journal of Transplantation
IS - 1
ER -