TY - JOUR
T1 - Balancing serendipity and reproducibility
T2 - Pluripotent stem cells as experimental systems for intellectual and developmental disorders
AU - Cross-IDDRC Human Stem Cell Working Group
AU - Anderson, Nickesha C.
AU - Chen, Pin Fang
AU - Meganathan, Kesavan
AU - Afshar Saber, Wardiya
AU - Petersen, Andrew J.
AU - Bhattacharyya, Anita
AU - Kroll, Kristen L.
AU - Sahin, Mustafa
N1 - Funding Information:
This study was made possible by the following IDDRC grants: U54HD090255 (BCH), U54HD087011 (Washington University), and U54HD090256 (Waisman Center, University of Wisconsin). M.S. was supported by R01NS113591 and U54NS092090 , N.C.A. was supported by T32MH112510 and the Tommy Fuss Fund, and W.A.S. was supported by a grant from the SSADH Foundation. K.L.K. and K.M. were supported by U01HG007530 (NINDS/ NIH Common Fund), U54HD087011 (administrative supplement), R01NS114551 , R01MH124808 , and P50HD129966 , and the M-CM Network, Jakob Gene Fund, and Washington University Center for Regenerative Medicine, Children's Discovery Institute (CDI-LI-2019-819), and Institute for Clinical and Translational Sciences. A.B. was supported by R03HD092640 , R21NS105339 , and U54HD087011 (administrative supplement), and grants from the John Merck Fund and the Jerome LeJeune Foundation.
Funding Information:
M.S. reports grant support from Novartis, Roche, Pfizer, Ipsen, LAM Therapeutics, and Quadrant Biosciences unrelated to this project. M.S. served on Scientific Advisory Boards for Sage, Roche, Celgene, Aeovian, Regenxbio, and Takeda. N.C.A., P.F.C., W.A.S., K.L.K., A.J.P., K.M., and A.B. report no competing interests.
Publisher Copyright:
© 2021 The Authors
PY - 2021/6/8
Y1 - 2021/6/8
N2 - Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) and their differentiation into neural lineages is a revolutionary experimental system for studying neurological disorders, including intellectual and developmental disabilities (IDDs). However, issues related to variability and reproducibility have hindered translating preclinical findings into drug discovery. Here, we identify areas for improvement by conducting a comprehensive review of 58 research articles that utilized iPSC-derived neural cells to investigate genetically defined IDDs. Based upon these findings, we propose recommendations for best practices that can be adopted by research scientists as well as journal editors.
AB - Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) and their differentiation into neural lineages is a revolutionary experimental system for studying neurological disorders, including intellectual and developmental disabilities (IDDs). However, issues related to variability and reproducibility have hindered translating preclinical findings into drug discovery. Here, we identify areas for improvement by conducting a comprehensive review of 58 research articles that utilized iPSC-derived neural cells to investigate genetically defined IDDs. Based upon these findings, we propose recommendations for best practices that can be adopted by research scientists as well as journal editors.
UR - http://www.scopus.com/inward/record.url?scp=85105014694&partnerID=8YFLogxK
U2 - 10.1016/j.stemcr.2021.03.025
DO - 10.1016/j.stemcr.2021.03.025
M3 - Review article
C2 - 33861989
AN - SCOPUS:85105014694
VL - 16
SP - 1446
EP - 1457
JO - Stem Cell Reports
JF - Stem Cell Reports
SN - 2213-6711
IS - 6
ER -