TY - JOUR
T1 - Balanced interhemispheric cortical activity is required for correct targeting of the corpus callosum
AU - Suárez, Rodrigo
AU - Fenlon, Laura R.
AU - Marek, Roger
AU - Avitan, Lilach
AU - Sah, Pankaj
AU - Goodhill, Geoffrey J.
AU - Richards, Linda J.
N1 - Funding Information:
We thank K. Deisseroth, A. Kriegstein, T. Saito, M. Stryker, and Y. Tagowa for providing DNA constructs; C. Wang, S. Liu, and J. Thomson for initial contributions to this project; L. Hammond for microscopy assistance; The University of Queensland Biological Resources and Queensland Brain Institute animal team for animal support; and R. Tweedale, P. Bartlett, J. Bertran-Gonzalez, and the Richards laboratory for input on the manuscript. This work was funded by National Health and Medical Research Council (NHMRC) project grants 1029975 and 1064174 (L.J.R.) 1043044 (G.J.G.) and by an Australian Research Council (ARC) Discovery project grant (P.S.). L.J.R. was supported by an NHMRC Principal Research Fellowship. P.S. was supported by an ARC Australian Professorial Fellowship. L.R.F. was supported by a University of Queensland Masters of Neuroscience scholarship and an Australian Postgraduate Award.
PY - 2014/6/18
Y1 - 2014/6/18
N2 - Bilateral integration of sensory and associative brain processing is achieved by precise connections between homologous regions in the two hemispheres via the corpus callosum. These connections form postnatally, and unilateral deprivation of sensory or spontaneous cortical activity during a critical period severely disrupts callosal wiring. However, little is known about how this early activity affects precise circuit formation. Here, using in utero electroporation of reporter genes, optogenetic constructs, and direct disruption of activity in callosal neurons combined with whisker ablations, we show that balanced interhemispheric activity, and not simply intact cortical activity in either hemisphere, is required for functional callosal targeting. Moreover, bilateral ablation of whiskers in symmetric or asymmetric configurations shows that spatially symmetric interhemispheric activity is required for appropriate callosal targeting. Our findings reveal a principle governing axon targeting, where spatially balanced activity between regions is required to establish their appropriate connectivity
AB - Bilateral integration of sensory and associative brain processing is achieved by precise connections between homologous regions in the two hemispheres via the corpus callosum. These connections form postnatally, and unilateral deprivation of sensory or spontaneous cortical activity during a critical period severely disrupts callosal wiring. However, little is known about how this early activity affects precise circuit formation. Here, using in utero electroporation of reporter genes, optogenetic constructs, and direct disruption of activity in callosal neurons combined with whisker ablations, we show that balanced interhemispheric activity, and not simply intact cortical activity in either hemisphere, is required for functional callosal targeting. Moreover, bilateral ablation of whiskers in symmetric or asymmetric configurations shows that spatially symmetric interhemispheric activity is required for appropriate callosal targeting. Our findings reveal a principle governing axon targeting, where spatially balanced activity between regions is required to establish their appropriate connectivity
UR - http://www.scopus.com/inward/record.url?scp=84902491207&partnerID=8YFLogxK
U2 - 10.1016/j.neuron.2014.04.040
DO - 10.1016/j.neuron.2014.04.040
M3 - Article
C2 - 24945772
AN - SCOPUS:84902491207
SN - 0896-6273
VL - 82
SP - 1289
EP - 1298
JO - Neuron
JF - Neuron
IS - 6
ER -