Bacterial macroscopic rope-like fibers with cytopathic and adhesive properties

Juan Xicohtencatl-Cortes, Zeus Saldaña, Wanyin Deng, Elsa Castañeda, Enrique Freer, Phil I. Tarr, B. Brett Finlay, José Luis Puente, Jorge A. Giro

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

We present a body of ultrastructural, biochemical, and genetic evidence that demonstrates the oligomerization of virulence-associated autotransporter proteins EspC or EspP produced by deadly human pathogens enterohemorrhagic and enteropathogenic Escherichia coli into novel macroscopic rope-like structures (>1 cm long). The rope-like structures showed high aggregation and insolubility, stability to anionic detergents and high temperature, and binding to Congo Red and thioflavin T dyes. These are properties also exhibited by human amyloidogenic proteins. These macroscopic ropes were not observed in cultures of nonpathogenic Escherichia coli or isogenic espP or espC deletion mutants of enterohemorrhagic or enteropathogenic Escherichia coli but were produced by an Escherichia coli K-12 strain carrying a plasmid expressing espP. Purified recombinant EspP monomers were able to self-assemble into macroscopic ropes upon incubation, suggesting that no other protein was required for assembly. The ropes bound to and showed cytopathic effects on cultured epithelial cells, served as a substratum for bacterial adherence and biofilm formation, and protected bacteria from antimicrobial compounds. We hypothesize that these ropes play a biologically significant role in the survival and pathogenic scheme of these organisms.

Original languageEnglish
Pages (from-to)32336-32342
Number of pages7
JournalJournal of Biological Chemistry
Volume285
Issue number42
DOIs
StatePublished - Oct 15 2010

Fingerprint

Dive into the research topics of 'Bacterial macroscopic rope-like fibers with cytopathic and adhesive properties'. Together they form a unique fingerprint.

Cite this