TY - JOUR
T1 - Bacteria from diverse habitats colonize and compete in the mouse gut
AU - Seedorf, Henning
AU - Griffin, Nicholas W.
AU - Ridaura, Vanessa K.
AU - Reyes, Alejandro
AU - Cheng, Jiye
AU - Rey, Federico E.
AU - Smith, Michelle I.
AU - Simon, Gabriel M.
AU - Scheffrahn, Rudolf H.
AU - Woebken, Dagmar
AU - Spormann, Alfred M.
AU - Van Treuren, William
AU - Ursell, Luke K.
AU - Pirrung, Megan
AU - Robbins-Pianka, Adam
AU - Cantarel, Brandi L.
AU - Lombard, Vincent
AU - Henrissat, Bernard
AU - Knight, Rob
AU - Gordon, Jeffrey I.
N1 - Funding Information:
We thank David O’Donnell, Maria Karlsson, Sabrina Wagoner, Janaki Guruge, Marty Meier, Jill Manchester, and Su Deng for superb technical assistance. This work was supported in part by grants from the NIH (DK30292, DK70977, DK78669, and DK58529) and the Crohn’s and Colitis Foundation of America. N.W.G. was supported by an NIH T32 postdoctoral fellowship training grant (DK007120), and L.K.U. was supported by a Signaling and Cellular Recognition Training grant (T32 GM08759). J.I.G. is cofounder of Matatu, Inc., a company that is characterizing the role of diet-by-microbiota interactions in defining health.
Publisher Copyright:
© 2014 Elsevier Inc.
PY - 2014/10/9
Y1 - 2014/10/9
N2 - To study how microbes establish themselves in a mammalian gut environment, we colonized germ-free mice with microbial communities from human, zebrafish, and termite guts, human skin and tongue, soil, and estuarine microbial mats. Bacteria from these foreign environments colonized and persisted in the mouse gut; their capacity to metabolize dietary and host carbohydrates and bile acids correlated with colonization success. Cohousing mice harboring these xenomicrobiota or a mouse cecal microbiota, along with germ-free "bystanders," revealed the success of particular bacterial taxa in invading guts with established communities and empty gut habitats. Unanticipated patterns of ecological succession were observed; for example, a soil-derived bacterium dominated even in the presence of bacteria from other gut communities (zebrafish and termite), and human-derived bacteria colonized germ-free bystander mice before mouse-derived organisms. This approach can be generalized to address a variety of mechanistic questions about succession, including succession in the context of microbiota-directed therapeutics.
AB - To study how microbes establish themselves in a mammalian gut environment, we colonized germ-free mice with microbial communities from human, zebrafish, and termite guts, human skin and tongue, soil, and estuarine microbial mats. Bacteria from these foreign environments colonized and persisted in the mouse gut; their capacity to metabolize dietary and host carbohydrates and bile acids correlated with colonization success. Cohousing mice harboring these xenomicrobiota or a mouse cecal microbiota, along with germ-free "bystanders," revealed the success of particular bacterial taxa in invading guts with established communities and empty gut habitats. Unanticipated patterns of ecological succession were observed; for example, a soil-derived bacterium dominated even in the presence of bacteria from other gut communities (zebrafish and termite), and human-derived bacteria colonized germ-free bystander mice before mouse-derived organisms. This approach can be generalized to address a variety of mechanistic questions about succession, including succession in the context of microbiota-directed therapeutics.
UR - http://www.scopus.com/inward/record.url?scp=84916884469&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2014.09.008
DO - 10.1016/j.cell.2014.09.008
M3 - Article
C2 - 25284151
AN - SCOPUS:84916884469
SN - 0092-8674
VL - 159
SP - 253
EP - 266
JO - Cell
JF - Cell
IS - 2
ER -