TY - JOUR
T1 - B lymphocytes inhibit human osteoclastogenesis by secretion of TGFβ
AU - Weitzmann, M. Neale
AU - Cenci, Simone
AU - Haug, Jeff
AU - Brown, Chris
AU - DiPersio, John
AU - Pacifici, Roberto
PY - 2000
Y1 - 2000
N2 - The role of B lymphocytes in osteoclast (OC) formation is controversial, because both stimulatory and inhibitory effects of B-lineage cells on osteoclastogenesis and life span have been reported. In this study, we have investigated the effects of mature B cells on human osteoclastogenesis using cultures of peripheral blood stem cells (PBSC), a system that generates functional OCs in the absence of stromal cells. We report that B cells inhibit the formation of OCs and shorten the life span of mature OCs by secreting transforming growth factor β (TGFβ), a factor that induces apoptosis in these cells. The antiosteoclastogenic effects of B cells are abolished by addition of anti-TGFβ antibody to osteoclast cultures and mimicked by treatment of B cell-deprived PBSC cultures with recombinant TGFβ, thus confirming TGFβ as the B cell produced antiosteoclastogenic activity. Thus, the ability of B cells to downregulate osteoclastogenesis by secretion of the apoptotic cytokine TGFβ provides new insights into the ability of immune cells to regulate OC formation under basal and inflammatory conditions. (C) 2000 Wiley-Liss, Inc.
AB - The role of B lymphocytes in osteoclast (OC) formation is controversial, because both stimulatory and inhibitory effects of B-lineage cells on osteoclastogenesis and life span have been reported. In this study, we have investigated the effects of mature B cells on human osteoclastogenesis using cultures of peripheral blood stem cells (PBSC), a system that generates functional OCs in the absence of stromal cells. We report that B cells inhibit the formation of OCs and shorten the life span of mature OCs by secreting transforming growth factor β (TGFβ), a factor that induces apoptosis in these cells. The antiosteoclastogenic effects of B cells are abolished by addition of anti-TGFβ antibody to osteoclast cultures and mimicked by treatment of B cell-deprived PBSC cultures with recombinant TGFβ, thus confirming TGFβ as the B cell produced antiosteoclastogenic activity. Thus, the ability of B cells to downregulate osteoclastogenesis by secretion of the apoptotic cytokine TGFβ provides new insights into the ability of immune cells to regulate OC formation under basal and inflammatory conditions. (C) 2000 Wiley-Liss, Inc.
KW - Cytokines
KW - Human osteoclastogenesis
KW - Lymphocytes
KW - TGFβ
UR - http://www.scopus.com/inward/record.url?scp=0034048059&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-4644(20000801)78:2<318::AID-JCB13>3.0.CO;2-N
DO - 10.1002/(SICI)1097-4644(20000801)78:2<318::AID-JCB13>3.0.CO;2-N
M3 - Article
C2 - 10842325
AN - SCOPUS:0034048059
SN - 0730-2312
VL - 78
SP - 318
EP - 324
JO - Journal of cellular biochemistry
JF - Journal of cellular biochemistry
IS - 2
ER -