"Axitinib affects cell viability and migration of a primary foetal lung adenocarcinoma culture"

Cecilia Menna; Elena De Falco; Luca Pacini; Gaia Scafetta; Paola Ruggieri; Rosa Puca; Vincenzo Petrozza; Anna Maria Ciccone; Erino Angelo Rendina; Antonella Calogero; Mohsen Ibrahim

doi:10.3109/07357907.2013.861472

"Axitinib affects cell viability and migration of a primary foetal lung adenocarcinoma culture"

Cecilia Menna
, Elena De Falco
, Luca Pacini
, Gaia Scafetta
, Paola Ruggieri
, Rosa Puca
, Vincenzo Petrozza
, Anna Maria Ciccone
, Erino Angelo Rendina
, Antonella Calogero
, Mohsen Ibrahim

Section of Thoracic Surgery

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Fetal lung adenocarcinoma (FLAC) is a rare variant of lung adenocarcinoma. Studies regarding FLAC have been based only on histopathological observations, thus representative in vitro models of FLAC cultures are unavailable. We have established and characterized a human primary FLAC cell culture, exploring its biology, chemosensitivity, and migration. FLAC cells and specimen showed significant upregulation of VEGF165 and HIF-1α mRNA levels. This observation was confirmed by in vitro chemosensitivity and migration assay, showing that only Axitinib was comparable to Cisplatin treatment. We provide a suitable in vitro model to further investigate the nature of this rare type of cancer.

Original language	English
Pages (from-to)	13-21
Number of pages	9
Journal	Cancer Investigation
Volume	32
Issue number	1
DOIs	https://doi.org/10.3109/07357907.2013.861472
State	Published - Jan 2014

Keywords

Antineoplastic drugs
Axitinib
Cancer primary cell cultures
Fetal lung adenocarcinoma
Lung adenocarcinoma
Vascular endothelial growth factor

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10.3109/07357907.2013.861472

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title = "{"}Axitinib affects cell viability and migration of a primary foetal lung adenocarcinoma culture{"}",

abstract = "Fetal lung adenocarcinoma (FLAC) is a rare variant of lung adenocarcinoma. Studies regarding FLAC have been based only on histopathological observations, thus representative in vitro models of FLAC cultures are unavailable. We have established and characterized a human primary FLAC cell culture, exploring its biology, chemosensitivity, and migration. FLAC cells and specimen showed significant upregulation of VEGF165 and HIF-1α mRNA levels. This observation was confirmed by in vitro chemosensitivity and migration assay, showing that only Axitinib was comparable to Cisplatin treatment. We provide a suitable in vitro model to further investigate the nature of this rare type of cancer.",

keywords = "Antineoplastic drugs, Axitinib, Cancer primary cell cultures, Fetal lung adenocarcinoma, Lung adenocarcinoma, Vascular endothelial growth factor",

author = "Cecilia Menna and \{De Falco\}, Elena and Luca Pacini and Gaia Scafetta and Paola Ruggieri and Rosa Puca and Vincenzo Petrozza and Ciccone, \{Anna Maria\} and Rendina, \{Erino Angelo\} and Antonella Calogero and Mohsen Ibrahim",

note = "Funding Information: This work was financially supported by the University of Rome “Sapienza.”",

year = "2014",

month = jan,

doi = "10.3109/07357907.2013.861472",

language = "English",

volume = "32",

pages = "13--21",

journal = "Cancer Investigation",

issn = "0735-7907",

number = "1",

}

TY - JOUR

T1 - "Axitinib affects cell viability and migration of a primary foetal lung adenocarcinoma culture"

AU - Menna, Cecilia

AU - De Falco, Elena

AU - Pacini, Luca

AU - Scafetta, Gaia

AU - Ruggieri, Paola

AU - Puca, Rosa

AU - Petrozza, Vincenzo

AU - Ciccone, Anna Maria

AU - Rendina, Erino Angelo

AU - Calogero, Antonella

AU - Ibrahim, Mohsen

N1 - Funding Information: This work was financially supported by the University of Rome “Sapienza.”

PY - 2014/1

Y1 - 2014/1

N2 - Fetal lung adenocarcinoma (FLAC) is a rare variant of lung adenocarcinoma. Studies regarding FLAC have been based only on histopathological observations, thus representative in vitro models of FLAC cultures are unavailable. We have established and characterized a human primary FLAC cell culture, exploring its biology, chemosensitivity, and migration. FLAC cells and specimen showed significant upregulation of VEGF165 and HIF-1α mRNA levels. This observation was confirmed by in vitro chemosensitivity and migration assay, showing that only Axitinib was comparable to Cisplatin treatment. We provide a suitable in vitro model to further investigate the nature of this rare type of cancer.

AB - Fetal lung adenocarcinoma (FLAC) is a rare variant of lung adenocarcinoma. Studies regarding FLAC have been based only on histopathological observations, thus representative in vitro models of FLAC cultures are unavailable. We have established and characterized a human primary FLAC cell culture, exploring its biology, chemosensitivity, and migration. FLAC cells and specimen showed significant upregulation of VEGF165 and HIF-1α mRNA levels. This observation was confirmed by in vitro chemosensitivity and migration assay, showing that only Axitinib was comparable to Cisplatin treatment. We provide a suitable in vitro model to further investigate the nature of this rare type of cancer.

KW - Antineoplastic drugs

KW - Axitinib

KW - Cancer primary cell cultures

KW - Fetal lung adenocarcinoma

KW - Lung adenocarcinoma

KW - Vascular endothelial growth factor

UR - https://www.scopus.com/pages/publications/84891525458

U2 - 10.3109/07357907.2013.861472

DO - 10.3109/07357907.2013.861472

M3 - Article

C2 - 24380379

AN - SCOPUS:84891525458

SN - 0735-7907

VL - 32

SP - 13

EP - 21

JO - Cancer Investigation

JF - Cancer Investigation

IS - 1

ER -

"Axitinib affects cell viability and migration of a primary foetal lung adenocarcinoma culture"

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Keywords

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