"Axitinib affects cell viability and migration of a primary foetal lung adenocarcinoma culture"

Cecilia Menna; Elena De Falco; Luca Pacini; Gaia Scafetta; Paola Ruggieri; Rosa Puca; Vincenzo Petrozza; Anna Maria Ciccone; Erino Angelo Rendina; Antonella Calogero; Mohsen Ibrahim

doi:10.3109/07357907.2013.861472

"Axitinib affects cell viability and migration of a primary foetal lung adenocarcinoma culture"

Cecilia Menna, Elena De Falco, Luca Pacini, Gaia Scafetta, Paola Ruggieri, Rosa Puca, Vincenzo Petrozza, Anna Maria Ciccone, Erino Angelo Rendina, Antonella Calogero, Mohsen Ibrahim

Section of Thoracic Surgery

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Fetal lung adenocarcinoma (FLAC) is a rare variant of lung adenocarcinoma. Studies regarding FLAC have been based only on histopathological observations, thus representative in vitro models of FLAC cultures are unavailable. We have established and characterized a human primary FLAC cell culture, exploring its biology, chemosensitivity, and migration. FLAC cells and specimen showed significant upregulation of VEGF165 and HIF-1α mRNA levels. This observation was confirmed by in vitro chemosensitivity and migration assay, showing that only Axitinib was comparable to Cisplatin treatment. We provide a suitable in vitro model to further investigate the nature of this rare type of cancer.

Original language	English
Pages (from-to)	13-21
Number of pages	9
Journal	Cancer Investigation
Volume	32
Issue number	1
DOIs	https://doi.org/10.3109/07357907.2013.861472
State	Published - Jan 2014

Keywords

Antineoplastic drugs
Axitinib
Cancer primary cell cultures
Fetal lung adenocarcinoma
Lung adenocarcinoma
Vascular endothelial growth factor

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10.3109/07357907.2013.861472

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title = "{"}Axitinib affects cell viability and migration of a primary foetal lung adenocarcinoma culture{"}",

abstract = "Fetal lung adenocarcinoma (FLAC) is a rare variant of lung adenocarcinoma. Studies regarding FLAC have been based only on histopathological observations, thus representative in vitro models of FLAC cultures are unavailable. We have established and characterized a human primary FLAC cell culture, exploring its biology, chemosensitivity, and migration. FLAC cells and specimen showed significant upregulation of VEGF165 and HIF-1α mRNA levels. This observation was confirmed by in vitro chemosensitivity and migration assay, showing that only Axitinib was comparable to Cisplatin treatment. We provide a suitable in vitro model to further investigate the nature of this rare type of cancer.",

keywords = "Antineoplastic drugs, Axitinib, Cancer primary cell cultures, Fetal lung adenocarcinoma, Lung adenocarcinoma, Vascular endothelial growth factor",

author = "Cecilia Menna and {De Falco}, Elena and Luca Pacini and Gaia Scafetta and Paola Ruggieri and Rosa Puca and Vincenzo Petrozza and Ciccone, {Anna Maria} and Rendina, {Erino Angelo} and Antonella Calogero and Mohsen Ibrahim",

note = "Funding Information: This work was financially supported by the University of Rome “Sapienza.”",

year = "2014",

month = jan,

doi = "10.3109/07357907.2013.861472",

language = "English",

volume = "32",

pages = "13--21",

journal = "Cancer Investigation",

issn = "0735-7907",

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T1 - "Axitinib affects cell viability and migration of a primary foetal lung adenocarcinoma culture"

AU - Menna, Cecilia

AU - De Falco, Elena

AU - Pacini, Luca

AU - Scafetta, Gaia

AU - Ruggieri, Paola

AU - Puca, Rosa

AU - Petrozza, Vincenzo

AU - Ciccone, Anna Maria

AU - Rendina, Erino Angelo

AU - Calogero, Antonella

AU - Ibrahim, Mohsen

N1 - Funding Information: This work was financially supported by the University of Rome “Sapienza.”

PY - 2014/1

Y1 - 2014/1

N2 - Fetal lung adenocarcinoma (FLAC) is a rare variant of lung adenocarcinoma. Studies regarding FLAC have been based only on histopathological observations, thus representative in vitro models of FLAC cultures are unavailable. We have established and characterized a human primary FLAC cell culture, exploring its biology, chemosensitivity, and migration. FLAC cells and specimen showed significant upregulation of VEGF165 and HIF-1α mRNA levels. This observation was confirmed by in vitro chemosensitivity and migration assay, showing that only Axitinib was comparable to Cisplatin treatment. We provide a suitable in vitro model to further investigate the nature of this rare type of cancer.

AB - Fetal lung adenocarcinoma (FLAC) is a rare variant of lung adenocarcinoma. Studies regarding FLAC have been based only on histopathological observations, thus representative in vitro models of FLAC cultures are unavailable. We have established and characterized a human primary FLAC cell culture, exploring its biology, chemosensitivity, and migration. FLAC cells and specimen showed significant upregulation of VEGF165 and HIF-1α mRNA levels. This observation was confirmed by in vitro chemosensitivity and migration assay, showing that only Axitinib was comparable to Cisplatin treatment. We provide a suitable in vitro model to further investigate the nature of this rare type of cancer.

KW - Antineoplastic drugs

KW - Axitinib

KW - Cancer primary cell cultures

KW - Fetal lung adenocarcinoma

KW - Lung adenocarcinoma

KW - Vascular endothelial growth factor

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DO - 10.3109/07357907.2013.861472

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JF - Cancer Investigation

IS - 1

ER -

"Axitinib affects cell viability and migration of a primary foetal lung adenocarcinoma culture"

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Keywords

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