Autoradiography of [3H]U-69593 binding sites in rat brain: evidence for κ opioid receptor subtypes

Bruce Nock, Atul Rajpara, Lynn H. O'Connor, Theodore J. Cicero

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88 Scopus citations

Abstract

In vitro quantiative autoradiography was used to determine the distribution of [3H]U-69593 binding sites in rat brain. The highest density of binding was found in areas of the tel- and diencephalon while certain areas of the mes- and metencephalon were moderately labeled. The distribution of [3H]U-69593 binding sites corresponds closely (but not precisely) to the distribution of sites labeled by [3H]EKC and [3H]bremazocine at room temperature but differs substantially from the distribution of sites labeled by [3H]EKC at 4°C (as described by others). These anatomical findings support previous biochemical evidence indicating that [3H]U-69593 selectively labels a κ opioid receptor subtype with characteristics that differ from the κ receptors labeled by [3H]EKC at 4°C.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalEuropean Journal of Pharmacology
Volume154
Issue number1
DOIs
StatePublished - Sep 1 1988

Keywords

  • [H]Bremazocine
  • [H]Ethylketocyclazpcine
  • [H]U-69593
  • [H]U-69593 autoradiography
  • κ Receptor subtypes

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