Autophagic flux and oxidative capacity of skeletal muscles during acute starvation

Mahroo Mofarrahi, Yeting Guo, Jeffrey A. Haspel, Augustine M.K. Choi, Elaine C. Davis, Gilles Gouspillou, Russell T. Hepple, Richard Godin, Yan Burelle, Sabah N.A. Hussain

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

ACTB, actin, beta; AKT, v-akt murine thymoma viral oncogene homolog; AMPK, 5'AMP-activated protein kinase; ANOVA, analysis of variance; ATG4B, autophagy-related 4B, cysteine peptidase; ATG5, autophagy-related 5; ATG7, autophagy-related 7; ATG12, autophagy-related 12; ATG13, autophagy-related 13; ATG14, autophagy-related 14; BCL2/ PPP1R50, B-cell CLL/lymphoma 2; BCL2L11/BIM, BCL2-like 11 (apoptosis facilitator); BECN1, Beclin 1, autophagy related; BNIP3, BCL2/adenovirus E1B 19 kDa interacting protein 3; BNIP3L/NIX, BCL2/adenovirus E1B 19 kDa interacting protein 3-like; DIA, diaphragm; EDL, extensor digitorium longus; EDTA, ethylenediaminetetraacetic acid; DTT, dithioreitol; FBXO32/ ATROGIN1, F-box protein 32; FOXO1, forkhead box O1; FOXO3, forkhead box O3; FOXO4, forkhead box O4; GABARAPL1, GABA(A) receptor-associated protein like 1; GP, gastrocnemius-plantaris; i.p., intraperitoneal; LAMP2A, lysosomal-associated membrane protein 2A; MAP1LC3B/LC3B, microtubule-associated protein 1 light chain 3 beta; MTORC1, mechanistic target of rapamycin complex 1; PARK2, parkinson protein 2, E3 ubiquitin protein ligase (parkin); PBS, phosphate-buffered saline; PIK3C3, phosphatidylinositol 3-kinase, catalytic subunit type 3; PMSF, phenylmethylsulfonyl fluoride; PVDF, polyvinylidene difluoride ; RB1CC1/FIP200, RB1-inducible coiled-coil 1; ROS, reactive oxygen species; RPS6, ribosomal protein S6; RPS6KB1, ribosomal protein S6 kinase, 70 kDa, polypeptide 1; SEM, standard error of the mean; SOD1, superoxide dismutase 1, soluble; SOL, soleus; SQSTM1/p62, sequestosome 1; TA, tibialis anterior; TRAF6/RNF85, TNF receptor-associated factor 6, E3 ubiquitin protein ligase; TUBB, tubulin, beta class I; ULK1, unc-51 like autophagy activating kinase 1; UVRAG, UV radiation-resistance associated.

Original languageEnglish
Pages (from-to)1604-1620
Number of pages17
JournalAutophagy
Volume9
Issue number10
DOIs
StatePublished - Oct 2013

Keywords

  • Autophagy
  • Fiber-type composition
  • Oxidative capacity
  • Skeletal muscles
  • Starvation

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