To meet a multiple-dose clinical evaluation of the P-gp modulation of [11C]morphine delivery into the human brain, radiosynthesis of [11C]morphine was accomplished on an automated system by N-methylation of normorphine with [11C]CH3I. A methodology employing optimized solid phase extraction of the HPLC eluent was developed. Radiosynthesis took 45min with a radiochemical yield ranging from 45% to 50% and specific activity ranging from 20 to 26Ci/γmol (decay corrected to end-of-bombardment); radiochemical and chemical purities were >95% (n=28).
- Automated radiosynthesis
- Positron emission tomography