Atherosclerotic neovasculature MR imaging with mixed manganese-gadolinium nanocolloids in hyperlipidemic rabbits

Kezheng Wang, Dipanjan Pan, Anne H. Schmieder, Angana Senpan, Shelton D. Caruthers, Grace Cui, John S. Allen, Huiying Zhang, Baozhong Shen, Gregory M. Lanza

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

A high r1 relaxivity manganese-gadolinium nanocolloid (αvβ3-MnOL-Gd NC) was developed and effectively detected atherosclerotic angiogenesis in rabbits fed cholesterol-rich diets for 12months using a clinical MRI scanner (3T). 3D mapping of neovasculature signal intensity revealed the spatial coherence and intensity of plaque angiogenic expansion, which may, with other high risk MR bioindicators, help identify high-risk patients with moderate (40% to 60%) vascular stenosis. Microscopy confirmed the predominant media and plaque distribution of fluorescent αvβ3-MnOL-Gd NC, mirroring the MR data. An expected close spatial association of αvβ3-integrin neovasculature and macrophages was noted, particularly within plaque shoulder regions. Manganese oleate bioelimination occurred via the biliary system into feces. Gd-DOTA was eliminated through the bile-fecal and renal excretion routes. αvβ3-MnOL-Gd NC offers an effective vehicle for T1w neovascular imaging in atherosclerosis.

Original languageEnglish
Pages (from-to)569-578
Number of pages10
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume11
Issue number3
DOIs
StatePublished - 2015

Keywords

  • Angiogenesis
  • Atherosclerosis
  • Contrast media
  • MRI
  • Molecular imaging
  • Nanotechnology

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