Assessment of Vascular Patterning in the Zebrafish

Amber N. Stratman, Brant M. Weinstein

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

The zebrafish has emerged as a valuable and important model organism for studying vascular development and vascular biology. Here, we discuss some of the approaches used to study vessels in fish, including loss-of-function tools such as morpholinos and genetic mutants, along with methods and considerations for assessing vascular phenotypes. We also provide detailed protocols for methods used for vital imaging of the zebrafish vasculature, including microangiography and long-term time-lapse imaging. The methods we describe, and the considerations we suggest using for assessing phenotypes observed using these methods, will help ensure reliable, valid conclusions when assessing vascular phenotypes following genetic or experimental manipulation of zebrafish.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages205-222
Number of pages18
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2206
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Blood vessel
  • Endothelium
  • Lymphatic vessel
  • Microangiography
  • Morpholino
  • Vascular biology
  • Vasculature
  • Zebrafish

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