Assessment of reproducibility of matrix-assisted laser desorption ionization - Time of flight mass spectrometry for bacterial and yeast identification

Lars F. Westblade; Omai B. Garner; Karen MacDonald; Constance Bradford; David H. Pincus; A. Brian Mochon; Rebecca Jennemann; Ryhana Manji; Maureen Bythrow; Michael A. Lewinski; Carey Ann D. Burnham; Christine C. Ginocchio

doi:10.1128/JCM.00187-15

Assessment of reproducibility of matrix-assisted laser desorption ionization - Time of flight mass spectrometry for bacterial and yeast identification

Lars F. Westblade, Omai B. Garner, Karen MacDonald, Constance Bradford, David H. Pincus, A. Brian Mochon, Rebecca Jennemann, Ryhana Manji, Maureen Bythrow, Michael A. Lewinski, Carey Ann D. Burnham, Christine C. Ginocchio

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21 Scopus citations

Abstract

Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has revolutionized the identification of clinical bacterial and yeast isolates. However, data describing the reproducibility of MALDI-TOF MS for microbial identification are scarce. In this study, we show that MALDI-TOF MS-based microbial identification is highly reproducible and can tolerate numerous variables, including differences in testing environments, instruments, operators, reagent lots, and sample positioning patterns. Finally, we reveal that samples of bacterial and yeast isolates prepared for MALDI-TOF MS identification can be repeatedly analyzed without compromising organism identification.

Original language	English
Pages (from-to)	2349-2352
Number of pages	4
Journal	Journal of clinical microbiology
Volume	53
Issue number	7
DOIs	https://doi.org/10.1128/JCM.00187-15
State	Published - Jul 1 2015

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Westblade, L. F., Garner, O. B., MacDonald, K., Bradford, C., Pincus, D. H., Mochon, A. B., Jennemann, R., Manji, R., Bythrow, M., Lewinski, M. A., Burnham, C. A. D., & Ginocchio, C. C. (2015). Assessment of reproducibility of matrix-assisted laser desorption ionization - Time of flight mass spectrometry for bacterial and yeast identification. Journal of clinical microbiology, 53(7), 2349-2352. https://doi.org/10.1128/JCM.00187-15

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abstract = "Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has revolutionized the identification of clinical bacterial and yeast isolates. However, data describing the reproducibility of MALDI-TOF MS for microbial identification are scarce. In this study, we show that MALDI-TOF MS-based microbial identification is highly reproducible and can tolerate numerous variables, including differences in testing environments, instruments, operators, reagent lots, and sample positioning patterns. Finally, we reveal that samples of bacterial and yeast isolates prepared for MALDI-TOF MS identification can be repeatedly analyzed without compromising organism identification.",

author = "Westblade, {Lars F.} and Garner, {Omai B.} and Karen MacDonald and Constance Bradford and Pincus, {David H.} and Mochon, {A. Brian} and Rebecca Jennemann and Ryhana Manji and Maureen Bythrow and Lewinski, {Michael A.} and Burnham, {Carey Ann D.} and Ginocchio, {Christine C.}",

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doi = "10.1128/JCM.00187-15",

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Westblade, LF, Garner, OB, MacDonald, K, Bradford, C, Pincus, DH, Mochon, AB, Jennemann, R, Manji, R, Bythrow, M, Lewinski, MA, Burnham, CAD & Ginocchio, CC 2015, 'Assessment of reproducibility of matrix-assisted laser desorption ionization - Time of flight mass spectrometry for bacterial and yeast identification', Journal of clinical microbiology, vol. 53, no. 7, pp. 2349-2352. https://doi.org/10.1128/JCM.00187-15

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T1 - Assessment of reproducibility of matrix-assisted laser desorption ionization - Time of flight mass spectrometry for bacterial and yeast identification

AU - Westblade, Lars F.

AU - Garner, Omai B.

AU - MacDonald, Karen

AU - Bradford, Constance

AU - Pincus, David H.

AU - Mochon, A. Brian

AU - Jennemann, Rebecca

AU - Manji, Ryhana

AU - Bythrow, Maureen

AU - Lewinski, Michael A.

AU - Burnham, Carey Ann D.

AU - Ginocchio, Christine C.

PY - 2015/7/1

Y1 - 2015/7/1

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AB - Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has revolutionized the identification of clinical bacterial and yeast isolates. However, data describing the reproducibility of MALDI-TOF MS for microbial identification are scarce. In this study, we show that MALDI-TOF MS-based microbial identification is highly reproducible and can tolerate numerous variables, including differences in testing environments, instruments, operators, reagent lots, and sample positioning patterns. Finally, we reveal that samples of bacterial and yeast isolates prepared for MALDI-TOF MS identification can be repeatedly analyzed without compromising organism identification.

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SN - 0095-1137

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SP - 2349

EP - 2352

JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

IS - 7

ER -

Assessment of reproducibility of matrix-assisted laser desorption ionization - Time of flight mass spectrometry for bacterial and yeast identification

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