Assessment of reproducibility of matrix-assisted laser desorption ionization - Time of flight mass spectrometry for bacterial and yeast identification

Lars F. Westblade, Omai B. Garner, Karen MacDonald, Constance Bradford, David H. Pincus, A. Brian Mochon, Rebecca Jennemann, Ryhana Manji, Maureen Bythrow, Michael A. Lewinski, Carey Ann D. Burnham, Christine C. Ginocchio

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has revolutionized the identification of clinical bacterial and yeast isolates. However, data describing the reproducibility of MALDI-TOF MS for microbial identification are scarce. In this study, we show that MALDI-TOF MS-based microbial identification is highly reproducible and can tolerate numerous variables, including differences in testing environments, instruments, operators, reagent lots, and sample positioning patterns. Finally, we reveal that samples of bacterial and yeast isolates prepared for MALDI-TOF MS identification can be repeatedly analyzed without compromising organism identification.

Original languageEnglish
Pages (from-to)2349-2352
Number of pages4
JournalJournal of clinical microbiology
Volume53
Issue number7
DOIs
StatePublished - Jul 1 2015

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