Assessment of postpreservation rat lung function using a new model for extended venous reperfusion

K. N. DeCampos, T. K. Waddell, A. S. Slutsky, M. Post, G. A. Patterson

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Understanding the physiological significance of biochemical events after lung preservation in rats has been hampered by the lack of a suitable model for physiological assessment. We have developed an ex vivo paracorporeal rat lung perfusion model that permits hemodynamic and gas exchange evaluation of lung function. After anesthesia and heparinization, the heart-lung block was removed and the left lung was reperfused for 1 h at a constant flow of 4 ml/min with homologous venous blood drained from the inferior vena cava of the paracorporeal (host) rat. The lung effluent was returned at the same flow rate to the host distal aorta. The model was validated by the assessment of lung function after room temperature ischemia. Animals were allocated into three groups (n = 6) according to the ischemic interval (group 1, 20 min; group 2, 3 h; group 3, 4 h). In groups 1 and 2 PO2, PCO2, mean airway pressure, and pulmonary arterial pressure were within the normal ranges and stable throughout the experiment. In contrast, lungs in group 3 demonstrated higher pulmonary arterial pressure and lower blood effluent PO2 than were found in either group 1 or 2. A significant weight gain during reperfusion was observed only in group 3 (4.23 ± 0.9 g; P < 0.002). For each lung, the final blood effluent PO2 correlated with the weight gain (R2 = 0.81; P < 0.0001). Our results indicate that this model can be used reliably to detect lung dysfunction after ischemic injury.

Original languageEnglish
Pages (from-to)1890-1896
Number of pages7
JournalJournal of Applied Physiology
Volume75
Issue number4
StatePublished - Jan 1 1993

Keywords

  • ex vivo lung reperfusion
  • gas exchange function
  • ischemic lung injury
  • lung preservation
  • rat paracorporeal model

Fingerprint

Dive into the research topics of 'Assessment of postpreservation rat lung function using a new model for extended venous reperfusion'. Together they form a unique fingerprint.

Cite this