TY - GEN
T1 - Assessment of particle associated bacteria in unchlorined drinking water from three treatment plants by cultivationindependent methods
AU - Liu, G.
AU - Ling, F. Q.
AU - Magic-Knezev, A.
AU - Liu, W. T.
AU - Verberk, J. Q.J.C.
PY - 2012
Y1 - 2012
N2 - Particle associated bacteria (PAB) in drinking water are of potential health significance due to their resistance to disinfection. In the current study, PAB from unchlorined drinking water of three treatment plants in the Netherlands were studied by cultivation-independent methods: Adenosine triphosphate (ATP) and total cell counts (TCC) with flow cytometry were used to quantify PAB, and high-throughput pyrosequencing was used to identify PAB. Scanning microscopy study confirmed the presence of multiple cells associated with single particle. The coupled energy dispersive spectroscopy found the particles were mainly consisted of Carbon, Oxygen, Silicon, Sodium, Calcium, Iron, Manganese. The number and activity of PAB ranged between 1.0-3.5×103 cells ml-1and 0.04-0.154 ng l -1 ATP and represented less than 2% of bacterial numbers (0.45-1.65 ×105 cells ml-1) and activity (1- 6 ng l -1 ATP) in water samples. These results indicate that a relatively low, but sufficient amount of PAB is present in drinking water. Furthermore, ATP per cell in PAB is higher than in planktonic bacteria indicating higher metabolic activity in PAB.
AB - Particle associated bacteria (PAB) in drinking water are of potential health significance due to their resistance to disinfection. In the current study, PAB from unchlorined drinking water of three treatment plants in the Netherlands were studied by cultivation-independent methods: Adenosine triphosphate (ATP) and total cell counts (TCC) with flow cytometry were used to quantify PAB, and high-throughput pyrosequencing was used to identify PAB. Scanning microscopy study confirmed the presence of multiple cells associated with single particle. The coupled energy dispersive spectroscopy found the particles were mainly consisted of Carbon, Oxygen, Silicon, Sodium, Calcium, Iron, Manganese. The number and activity of PAB ranged between 1.0-3.5×103 cells ml-1and 0.04-0.154 ng l -1 ATP and represented less than 2% of bacterial numbers (0.45-1.65 ×105 cells ml-1) and activity (1- 6 ng l -1 ATP) in water samples. These results indicate that a relatively low, but sufficient amount of PAB is present in drinking water. Furthermore, ATP per cell in PAB is higher than in planktonic bacteria indicating higher metabolic activity in PAB.
UR - http://www.scopus.com/inward/record.url?scp=84883079326&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883079326
SN - 9781627481328
T3 - 14th Water Distribution Systems Analysis Conference 2012, WDSA 2012
SP - 198
EP - 208
BT - 14th Water Distribution Systems Analysis Conference 2012, WDSA 2012
T2 - 14th Water Distribution Systems Analysis Conference 2012, WDSA 2012
Y2 - 24 September 2012 through 27 September 2012
ER -