@inbook{35647009fb904df2904f57104c5474e7,
title = "Assessment of NK cell metabolism",
abstract = "There has been increasing recognition of the importance of metabolism on immune cell differentiation, homeostasis, and function. Recently, our lab and others have begun to investigate the metabolic requirements for NK cell differentiation and activation. Here, we describe approaches for the in vitro assessment of NK cell metabolism. We present methods for using inhibitors to alter cellular metabolism, measurement of intracellular ATP in NK cells, assessment of real-time glycolysis and oxidative phosphorylation by an extracellular flux assay from Seahorse Biosciences, and some basic protocols for stimulation of NK cells via cytokines and receptors.",
keywords = "Glycolysis, Interferon-gamma, Metabolism, Natural killer cells, Oxidative phosphorylation",
author = "Keppel, {Molly P.} and Cooper, {Megan A.}",
note = "Funding Information: The authors thank Nermina Saucier for excellent technical contributions and Todd Fehniger for helpful discussion. Work in the Cooper laboratory was supported by the NIH/NIAID 1K08-AI085030, the Child Health Research Center at Washington University School of Medicine (K12-HD076224), and The Children{\textquoteright}s Discovery Institute and St. Louis Children{\textquoteright}s Hospital Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
doi = "10.1007/978-1-4939-3684-7_3",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "27--42",
booktitle = "Methods in Molecular Biology",
}