TY - GEN
T1 - Assessment of natriuretic peptide clearance receptor with positron emission tomography in cardiovascular disease models
AU - Liu, Yongjian
AU - Rossin, R.
AU - Abendschein, D.
AU - Woodard, G. E.
AU - Zheng, J.
AU - McCommis, K.
AU - Woodard, P. K.
AU - Welch, M. J.
PY - 2009/12/1
Y1 - 2009/12/1
N2 - Cardiovascular molecular imaging is a rapidly evolving field of research, aiming to image and quantify molecular and cellular targets in vivo. Natriuretic peptides (NPs) can acts as a potent inhibitor of vascular smooth muscle cell migration and proliferation through activation of the clearance receptor (NPR-C). In this study, the potential use of a C-type NP fragment (C-ANF) to image the NPR-C receptor expression in developing plaque-like lesions in a rabbit atherosclerosis model and surgically ligated thigh of murine hindlimb ischemia model by functionalization with 1,4,7,10-tetraazacyclododecane-1,4,7, 10-tetraacetic acid (DOTA) and labeled with copper-64 for non-invasive imaging with positron emission tomography (PET). Results clearly showed the significantly higher tracer uptake in the injured sites in both models compared to the contralateral control sites. PET imaging and competitive receptor blocking studies demonstrated the NPR-C receptor mediated tracer uptake.
AB - Cardiovascular molecular imaging is a rapidly evolving field of research, aiming to image and quantify molecular and cellular targets in vivo. Natriuretic peptides (NPs) can acts as a potent inhibitor of vascular smooth muscle cell migration and proliferation through activation of the clearance receptor (NPR-C). In this study, the potential use of a C-type NP fragment (C-ANF) to image the NPR-C receptor expression in developing plaque-like lesions in a rabbit atherosclerosis model and surgically ligated thigh of murine hindlimb ischemia model by functionalization with 1,4,7,10-tetraazacyclododecane-1,4,7, 10-tetraacetic acid (DOTA) and labeled with copper-64 for non-invasive imaging with positron emission tomography (PET). Results clearly showed the significantly higher tracer uptake in the injured sites in both models compared to the contralateral control sites. PET imaging and competitive receptor blocking studies demonstrated the NPR-C receptor mediated tracer uptake.
UR - http://www.scopus.com/inward/record.url?scp=77952723614&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:77952723614
SN - 9781424472819
T3 - Computers in Cardiology
SP - 241
EP - 244
BT - Computers in Cardiology 2009, CinC 2009
Y2 - 13 September 2009 through 16 September 2009
ER -