Assessment of collagen changes in ovarian tissue by extracting optical scattering coefficient from OCT images

Yi Yang; Tianheng Wang; Nrusingh Biswal; Xiaohong Wang; Melinda Sanders; Molly Brewer; Quing Zhu

doi:10.1117/12.909535

Assessment of collagen changes in ovarian tissue by extracting optical scattering coefficient from OCT images

Yi Yang
, Tianheng Wang
, Nrusingh Biswal
, Xiaohong Wang
, Melinda Sanders
, Molly Brewer
, Quing Zhu

Department of Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Optical scattering coefficient from ex-vivo unfixed normal and malignant ovarian tissue was quantitatively extracted by fitting optical coherence tomography (OCT) A-line signals to a single scattering model. 1097 average A-line measurements at a wavelength of 1310nm were performed at 108 sites obtained from 18 ovaries. The average scattering coefficient obtained from normal group consisted of 833 measurements from 88 sites was 2.41 mm¹ (±0.59), while the average coefficient obtained from malignant group consisted of 264 measurements from 20 sites was 1.55 mm¹ (±0.46). Using a threshold of 2 mm¹ for each ovary, a sensitivity of 100% and a specificity of 100% were achieved. The amount of collagen within OCT imaging depth was analyzed from the tissue histological section stained with Sirius Red. The average collagen area fraction (CAF) obtained from normal group was 48.4% (±12.3%), while the average CAF obtained from malignant group was 11.4% (±4.7%). Statistical significance of the collagen content was found between the two groups (p < 0.001). The preliminary data demonstrated that quantitative extraction of optical scattering coefficient from OCT images could be a potential powerful method for ovarian cancer detection and diagnosis.

Original language	English
Title of host publication	Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVI
Publisher	SPIE
ISBN (Print)	9780819488565
DOIs	https://doi.org/10.1117/12.909535
State	Published - 2012
Event	Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVI - San Francisco, CA, United States Duration: Jan 23 2012 → Jan 25 2012

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	8213
ISSN (Print)	1605-7422

Conference

Conference	Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVI
Country/Territory	United States
City	San Francisco, CA
Period	01/23/12 → 01/25/12

Keywords

Collagen
Optical coherence tomography
Ovarian cancer
Scattering coefficient
Single scattering model

Access to Document

10.1117/12.909535

Cite this

Yang, Y., Wang, T., Biswal, N., Wang, X., Sanders, M., Brewer, M., & Zhu, Q. (2012). Assessment of collagen changes in ovarian tissue by extracting optical scattering coefficient from OCT images. In Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVI Article 82130C (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8213). SPIE. https://doi.org/10.1117/12.909535

@inproceedings{479e139b0f8f44338f8cf76498c1d74e,

title = "Assessment of collagen changes in ovarian tissue by extracting optical scattering coefficient from OCT images",

abstract = "Optical scattering coefficient from ex-vivo unfixed normal and malignant ovarian tissue was quantitatively extracted by fitting optical coherence tomography (OCT) A-line signals to a single scattering model. 1097 average A-line measurements at a wavelength of 1310nm were performed at 108 sites obtained from 18 ovaries. The average scattering coefficient obtained from normal group consisted of 833 measurements from 88 sites was 2.41 mm1 (±0.59), while the average coefficient obtained from malignant group consisted of 264 measurements from 20 sites was 1.55 mm1 (±0.46). Using a threshold of 2 mm1 for each ovary, a sensitivity of 100\% and a specificity of 100\% were achieved. The amount of collagen within OCT imaging depth was analyzed from the tissue histological section stained with Sirius Red. The average collagen area fraction (CAF) obtained from normal group was 48.4\% (±12.3\%), while the average CAF obtained from malignant group was 11.4\% (±4.7\%). Statistical significance of the collagen content was found between the two groups (p < 0.001). The preliminary data demonstrated that quantitative extraction of optical scattering coefficient from OCT images could be a potential powerful method for ovarian cancer detection and diagnosis.",

keywords = "Collagen, Optical coherence tomography, Ovarian cancer, Scattering coefficient, Single scattering model",

author = "Yi Yang and Tianheng Wang and Nrusingh Biswal and Xiaohong Wang and Melinda Sanders and Molly Brewer and Quing Zhu",

year = "2012",

doi = "10.1117/12.909535",

language = "English",

isbn = "9780819488565",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

booktitle = "Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVI",

note = "Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVI ; Conference date: 23-01-2012 Through 25-01-2012",

}

Yang, Y, Wang, T, Biswal, N, Wang, X, Sanders, M, Brewer, M & Zhu, Q 2012, Assessment of collagen changes in ovarian tissue by extracting optical scattering coefficient from OCT images. in Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVI., 82130C, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8213, SPIE, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVI, San Francisco, CA, United States, 01/23/12. https://doi.org/10.1117/12.909535

Assessment of collagen changes in ovarian tissue by extracting optical scattering coefficient from OCT images. / Yang, Yi; Wang, Tianheng; Biswal, Nrusingh et al.
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVI. SPIE, 2012. 82130C (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8213).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Assessment of collagen changes in ovarian tissue by extracting optical scattering coefficient from OCT images

AU - Yang, Yi

AU - Wang, Tianheng

AU - Biswal, Nrusingh

AU - Wang, Xiaohong

AU - Sanders, Melinda

AU - Brewer, Molly

AU - Zhu, Quing

PY - 2012

Y1 - 2012

N2 - Optical scattering coefficient from ex-vivo unfixed normal and malignant ovarian tissue was quantitatively extracted by fitting optical coherence tomography (OCT) A-line signals to a single scattering model. 1097 average A-line measurements at a wavelength of 1310nm were performed at 108 sites obtained from 18 ovaries. The average scattering coefficient obtained from normal group consisted of 833 measurements from 88 sites was 2.41 mm1 (±0.59), while the average coefficient obtained from malignant group consisted of 264 measurements from 20 sites was 1.55 mm1 (±0.46). Using a threshold of 2 mm1 for each ovary, a sensitivity of 100% and a specificity of 100% were achieved. The amount of collagen within OCT imaging depth was analyzed from the tissue histological section stained with Sirius Red. The average collagen area fraction (CAF) obtained from normal group was 48.4% (±12.3%), while the average CAF obtained from malignant group was 11.4% (±4.7%). Statistical significance of the collagen content was found between the two groups (p < 0.001). The preliminary data demonstrated that quantitative extraction of optical scattering coefficient from OCT images could be a potential powerful method for ovarian cancer detection and diagnosis.

AB - Optical scattering coefficient from ex-vivo unfixed normal and malignant ovarian tissue was quantitatively extracted by fitting optical coherence tomography (OCT) A-line signals to a single scattering model. 1097 average A-line measurements at a wavelength of 1310nm were performed at 108 sites obtained from 18 ovaries. The average scattering coefficient obtained from normal group consisted of 833 measurements from 88 sites was 2.41 mm1 (±0.59), while the average coefficient obtained from malignant group consisted of 264 measurements from 20 sites was 1.55 mm1 (±0.46). Using a threshold of 2 mm1 for each ovary, a sensitivity of 100% and a specificity of 100% were achieved. The amount of collagen within OCT imaging depth was analyzed from the tissue histological section stained with Sirius Red. The average collagen area fraction (CAF) obtained from normal group was 48.4% (±12.3%), while the average CAF obtained from malignant group was 11.4% (±4.7%). Statistical significance of the collagen content was found between the two groups (p < 0.001). The preliminary data demonstrated that quantitative extraction of optical scattering coefficient from OCT images could be a potential powerful method for ovarian cancer detection and diagnosis.

KW - Collagen

KW - Optical coherence tomography

KW - Ovarian cancer

KW - Scattering coefficient

KW - Single scattering model

UR - https://www.scopus.com/pages/publications/84899056168

U2 - 10.1117/12.909535

DO - 10.1117/12.909535

M3 - Conference contribution

AN - SCOPUS:84899056168

SN - 9780819488565

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVI

PB - SPIE

T2 - Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVI

Y2 - 23 January 2012 through 25 January 2012

ER -

Yang Y, Wang T, Biswal N, Wang X, Sanders M, Brewer M et al. Assessment of collagen changes in ovarian tissue by extracting optical scattering coefficient from OCT images. In Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVI. SPIE. 2012. 82130C. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.909535

Assessment of collagen changes in ovarian tissue by extracting optical scattering coefficient from OCT images

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