Assessing the mouse intestinal microbiota in settings of type-2 immune responses

Mei San Tang, Rowann Bowcutt, P’ng Loke

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations


The microbial communities that reside within the mammalian host play important roles in the development of a robust host immune system. With the advent of sequencing technology and barcoding strategy of the bacterial 16S ribosomal RNA (rRNA) gene, microbiota studies are becoming more economical but also more important in many immunology studies. Here, we described a representative study protocol to characterize how the microbiota changes during an intestinal helminth infection, with emphasis on subtle aspects of the experimental design that are critical for data interpretation.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages12
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • 16S rRNA
  • Helminth
  • Microbiota
  • Type 2 immunity


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