TY - JOUR
T1 - Assessing the effects of mitofusin 2 deficiency in the adult heart using 3D electron tomography
AU - Kalkhoran, Siavash Beikoghli
AU - Hall, Andrew R.
AU - White, Ian J.
AU - Cooper, Jackie
AU - Fan, Qiao
AU - Ong, Sang Bing
AU - Hernández-Reséndiz, Sauri
AU - Cabrera-Fuentes, Hector
AU - Chinda, Kroekkiat
AU - Chakraborty, Bibhas
AU - Dorn, Gerald W.
AU - Yellon, Derek M.
AU - Hausenloy, Derek J.
N1 - Funding Information:
D. J. Hausenloy is supported by the Duke-National University Singapore Medical School, Singapore Ministry of Health’s National Medical Research Council under its Clinician Scientist-Senior Investigator Scheme (NMRC/ CSA-SI/0011/2017) and Collaborative Centre Grant Scheme (NMRC/CGAug16C006), the Singapore Ministry of Education Academic Research Fund Tier 2 (MOE2016-T2-2-021), the National Institute for Health Research University College London Hospitals Biomedical Research Centre, and the British Heart Foundation (FS/10/039/28270). A. R. Hall was funded by the Medical Research Council (grant number MR/J003530/1). I. J. White was supported by U.K. Medical Research Council core funding to the MRC-UCL LMCB University Unit (grant MC_U122665002). S. B. Ong is supported by the Singapore Ministry of Health’s National Medical Research Council under its Open
Publisher Copyright:
© 2017 The Authors.
PY - 2017/9/12
Y1 - 2017/9/12
N2 - The effects of mitofusin 2 (MFN2) deficiency, on mitochondrial morphology and the mitochondria-junctional sarcoplasmic reticulum (jSR) complex in the adult heart, have been previously investigated using 2D electron microscopy, an approach which is unable to provide a 3D spatial assessment of these imaging parameters. Here, we use 3D electron tomography to show that MFN2-deficient mitochondria are larger in volume, more elongated, and less rounded; have fewer mitochondria-jSR contacts, and an increase in the distance between mitochondria and jSR, when compared to WT mitochondria. In comparison to 2D electron microscopy, 3D electron tomography can provide further insights into mitochondrial morphology and the mitochondria- jSR complex in the adult heart.
AB - The effects of mitofusin 2 (MFN2) deficiency, on mitochondrial morphology and the mitochondria-junctional sarcoplasmic reticulum (jSR) complex in the adult heart, have been previously investigated using 2D electron microscopy, an approach which is unable to provide a 3D spatial assessment of these imaging parameters. Here, we use 3D electron tomography to show that MFN2-deficient mitochondria are larger in volume, more elongated, and less rounded; have fewer mitochondria-jSR contacts, and an increase in the distance between mitochondria and jSR, when compared to WT mitochondria. In comparison to 2D electron microscopy, 3D electron tomography can provide further insights into mitochondrial morphology and the mitochondria- jSR complex in the adult heart.
KW - 3D electron tomography
KW - MFN2 KO
KW - Mitochondria-jSR junction
UR - http://www.scopus.com/inward/record.url?scp=85029425656&partnerID=8YFLogxK
U2 - 10.14814/phy2.13437
DO - 10.14814/phy2.13437
M3 - Article
C2 - 28904083
AN - SCOPUS:85029425656
VL - 5
JO - Physiological Reports
JF - Physiological Reports
SN - 2051-817X
IS - 17
M1 - e13437
ER -