Assessing the effects of mitofusin 2 deficiency in the adult heart using 3D electron tomography

Siavash Beikoghli Kalkhoran; Andrew R. Hall; Ian J. White; Jackie Cooper; Qiao Fan; Sang Bing Ong; Sauri Hernández-Reséndiz; Hector Cabrera-Fuentes; Kroekkiat Chinda; Bibhas Chakraborty; Gerald W. Dorn; Derek M. Yellon; Derek J. Hausenloy

doi:10.14814/phy2.13437

Assessing the effects of mitofusin 2 deficiency in the adult heart using 3D electron tomography

Siavash Beikoghli Kalkhoran, Andrew R. Hall, Ian J. White, Jackie Cooper, Qiao Fan, Sang Bing Ong, Sauri Hernández-Reséndiz, Hector Cabrera-Fuentes, Kroekkiat Chinda, Bibhas Chakraborty, Gerald W. Dorn, Derek M. Yellon, Derek J. Hausenloy

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11 Scopus citations

Abstract

The effects of mitofusin 2 (MFN2) deficiency, on mitochondrial morphology and the mitochondria-junctional sarcoplasmic reticulum (jSR) complex in the adult heart, have been previously investigated using 2D electron microscopy, an approach which is unable to provide a 3D spatial assessment of these imaging parameters. Here, we use 3D electron tomography to show that MFN2-deficient mitochondria are larger in volume, more elongated, and less rounded; have fewer mitochondria-jSR contacts, and an increase in the distance between mitochondria and jSR, when compared to WT mitochondria. In comparison to 2D electron microscopy, 3D electron tomography can provide further insights into mitochondrial morphology and the mitochondria- jSR complex in the adult heart.

Original language	English
Article number	e13437
Journal	Physiological Reports
Volume	5
Issue number	17
DOIs	https://doi.org/10.14814/phy2.13437
State	Published - Sep 12 2017

Keywords

3D electron tomography
MFN2 KO
Mitochondria-jSR junction

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10.14814/phy2.13437

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Kalkhoran, S. B., Hall, A. R., White, I. J., Cooper, J., Fan, Q., Ong, S. B., Hernández-Reséndiz, S., Cabrera-Fuentes, H., Chinda, K., Chakraborty, B., Dorn, G. W., Yellon, D. M., & Hausenloy, D. J. (2017). Assessing the effects of mitofusin 2 deficiency in the adult heart using 3D electron tomography. Physiological Reports, 5(17), Article e13437. https://doi.org/10.14814/phy2.13437

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title = "Assessing the effects of mitofusin 2 deficiency in the adult heart using 3D electron tomography",

abstract = "The effects of mitofusin 2 (MFN2) deficiency, on mitochondrial morphology and the mitochondria-junctional sarcoplasmic reticulum (jSR) complex in the adult heart, have been previously investigated using 2D electron microscopy, an approach which is unable to provide a 3D spatial assessment of these imaging parameters. Here, we use 3D electron tomography to show that MFN2-deficient mitochondria are larger in volume, more elongated, and less rounded; have fewer mitochondria-jSR contacts, and an increase in the distance between mitochondria and jSR, when compared to WT mitochondria. In comparison to 2D electron microscopy, 3D electron tomography can provide further insights into mitochondrial morphology and the mitochondria- jSR complex in the adult heart.",

keywords = "3D electron tomography, MFN2 KO, Mitochondria-jSR junction",

author = "Kalkhoran, {Siavash Beikoghli} and Hall, {Andrew R.} and White, {Ian J.} and Jackie Cooper and Qiao Fan and Ong, {Sang Bing} and Sauri Hern{\'a}ndez-Res{\'e}ndiz and Hector Cabrera-Fuentes and Kroekkiat Chinda and Bibhas Chakraborty and Dorn, {Gerald W.} and Yellon, {Derek M.} and Hausenloy, {Derek J.}",

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AU - Kalkhoran, Siavash Beikoghli

AU - Hall, Andrew R.

AU - White, Ian J.

AU - Cooper, Jackie

AU - Fan, Qiao

AU - Ong, Sang Bing

AU - Hernández-Reséndiz, Sauri

AU - Cabrera-Fuentes, Hector

AU - Chinda, Kroekkiat

AU - Chakraborty, Bibhas

AU - Dorn, Gerald W.

AU - Yellon, Derek M.

AU - Hausenloy, Derek J.

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Y1 - 2017/9/12

N2 - The effects of mitofusin 2 (MFN2) deficiency, on mitochondrial morphology and the mitochondria-junctional sarcoplasmic reticulum (jSR) complex in the adult heart, have been previously investigated using 2D electron microscopy, an approach which is unable to provide a 3D spatial assessment of these imaging parameters. Here, we use 3D electron tomography to show that MFN2-deficient mitochondria are larger in volume, more elongated, and less rounded; have fewer mitochondria-jSR contacts, and an increase in the distance between mitochondria and jSR, when compared to WT mitochondria. In comparison to 2D electron microscopy, 3D electron tomography can provide further insights into mitochondrial morphology and the mitochondria- jSR complex in the adult heart.

AB - The effects of mitofusin 2 (MFN2) deficiency, on mitochondrial morphology and the mitochondria-junctional sarcoplasmic reticulum (jSR) complex in the adult heart, have been previously investigated using 2D electron microscopy, an approach which is unable to provide a 3D spatial assessment of these imaging parameters. Here, we use 3D electron tomography to show that MFN2-deficient mitochondria are larger in volume, more elongated, and less rounded; have fewer mitochondria-jSR contacts, and an increase in the distance between mitochondria and jSR, when compared to WT mitochondria. In comparison to 2D electron microscopy, 3D electron tomography can provide further insights into mitochondrial morphology and the mitochondria- jSR complex in the adult heart.

KW - 3D electron tomography

KW - MFN2 KO

KW - Mitochondria-jSR junction

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DO - 10.14814/phy2.13437

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AN - SCOPUS:85029425656

SN - 2051-817X

VL - 5

JO - Physiological Reports

JF - Physiological Reports

IS - 17

M1 - e13437

ER -

Assessing the effects of mitofusin 2 deficiency in the adult heart using 3D electron tomography

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Keywords

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