Assessing protein synthesis by platelets

Hansjörg Schwertz, Jesse W. Rowley, Neal D. Tolley, Robert A. Campbell, Andrew S. Weyrich

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

21 Scopus citations

Abstract

Platelets are anucleate cytoplasts that circulate in the bloodstream for approximately 9-11 days. Because they lack nuclei, platelets were considered incapable of protein synthesis. However, studies over the last decade have revealed that platelets use a variety of translational control pathways to synthesize proteins. A variety of protocols can be employed to assess protein synthesis by platelets. These protocols are scattered throughout the literature and, more often than not, lack critical details. In this chapter, we thoroughly outline methods used in our laboratory to assess protein synthesis by platelets.

Original languageEnglish
Title of host publicationPlatelets and Megakaryocytes
Subtitle of host publicationVolume 3, Additional Protocols and Perspectives
EditorsJonathan Gibbins, Martyn Mahaut-Smith
Pages141-153
Number of pages13
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume788
ISSN (Print)1064-3745

Keywords

  • MicroRNA processing
  • Platelet protein synthesis
  • Pre-mRNA processing
  • Protein Translation
  • Spliceosome factors

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