Natural killer (NK) cells express receptors to detect and kill target cells based on expression of target cell surface molecules. Through a process termed NK cell licensing, only NK cells that express inhibitory receptors (e.g., Ly49 receptors in the mouse) for self-major histocompatibility complex (MHC) class I molecules become functionally competent to be triggered through their activation receptors. To determine the licensing status of particular Ly49(+) murine NK cell subsets, splenocytes are stimulated with plate-bound anti-NK1.1 monoclonal antibody in the presence of brefeldin A and then assessed for NK cell activation on a single-cell basis using intracellular cytokine interferon-gamma staining and flow cytometry.

Original languageEnglish
Pages (from-to)39-49
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
StatePublished - 2010


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