Assessing DNA Damage Responses Using B Lymphocyte Cultures

Rachel Johnston, Lynn S. White, Jeffrey J. Bednarski

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Development of B cells requires the programmed generation and repair of double-stranded DNA breaks in antigen receptor genes. Investigation of the cellular responses to these DNA breaks has established important insights into B cell development and, more broadly, has provided fundamental advances into the molecular mechanisms of DNA damage response pathways. Abelson transformed pre-B cell lines and primary pre-B cell cultures are malleable experimental systems with diverse applications for studying DNA damage responses. This chapter describes methods for generating these cellular systems, inducing and quantifying DSBs, and assessing DNA damage programs.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages69-80
Number of pages12
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2444
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • B cells
  • DNA damage response
  • DNA double-stranded breaks
  • V(D)J recombination

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