Assays for Monitoring Toxoplasma gondii Infectivity in the Laboratory Mouse

Qiuling Wang, L. David Sibley

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Toxoplasma is a widespread parasite of animals including many rodents that are a natural part of the transmission cycle between cats, which serve as the definitive host. Although wild rodents, including house mice, are relatively resistant, laboratory mice are highly susceptible to infection. As such, laboratory mice have been used to compare pathogenesis of natural variants and to evaluate the contributions of both host and parasite genes to infection. Protocols are provided here for evaluating acute and chronic infection with different parasite strains in laboratory mice. These protocols should provide uniform standards for evaluating natural variants and attenuated mutants and for comparing outcomes across different studies and between different laboratories.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages99-116
Number of pages18
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2071
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Bioluminescence
  • Central nervous system
  • Dissemination
  • Pathogenesis
  • Virulence

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    Wang, Q., & Sibley, L. D. (2020). Assays for Monitoring Toxoplasma gondii Infectivity in the Laboratory Mouse. In Methods in Molecular Biology (pp. 99-116). (Methods in Molecular Biology; Vol. 2071). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9857-9_5