Assays for eukaryotic cell chemotaxis

Zac Pujic; Duncan Mortimer; Julia Feldner; Geoffrey J. Goodhill

doi:10.2174/138620709788681952

Assays for eukaryotic cell chemotaxis

Zac Pujic, Duncan Mortimer, Julia Feldner, Geoffrey J. Goodhill

Research output: Contribution to journal › Review article › peer-review

19 Scopus citations

Abstract

Chemotaxis is essential for many biological processes. Much of our understanding of the mechanisms underlying chemotaxis is based on a variety of in vitro assays. We review these assays, dividing them into groups depending on the process used to generate the gradient. We describe how each method works, its strengths and limitations, and provide some information about the kinds of cells that have been studied with each assay.

Original language	English
Pages (from-to)	580-588
Number of pages	9
Journal	Combinatorial Chemistry and High Throughput Screening
Volume	12
Issue number	6
DOIs	https://doi.org/10.2174/138620709788681952
State	Published - Jul 2009

Keywords

Axon guidance
Boyden
Cell migration
Diffusion
Gradient
Invasion assay
Metastasis
Microfluidics

Access to Document

10.2174/138620709788681952

Cite this

@article{5d1df402cc2041849beb2bef957226d7,

title = "Assays for eukaryotic cell chemotaxis",

abstract = "Chemotaxis is essential for many biological processes. Much of our understanding of the mechanisms underlying chemotaxis is based on a variety of in vitro assays. We review these assays, dividing them into groups depending on the process used to generate the gradient. We describe how each method works, its strengths and limitations, and provide some information about the kinds of cells that have been studied with each assay.",

keywords = "Axon guidance, Boyden, Cell migration, Diffusion, Gradient, Invasion assay, Metastasis, Microfluidics",

author = "Zac Pujic and Duncan Mortimer and Julia Feldner and Goodhill, {Geoffrey J.}",

year = "2009",

month = jul,

doi = "10.2174/138620709788681952",

language = "English",

volume = "12",

pages = "580--588",

journal = "Combinatorial Chemistry and High Throughput Screening",

issn = "1386-2073",

number = "6",

}

TY - JOUR

T1 - Assays for eukaryotic cell chemotaxis

AU - Pujic, Zac

AU - Mortimer, Duncan

AU - Feldner, Julia

AU - Goodhill, Geoffrey J.

PY - 2009/7

Y1 - 2009/7

N2 - Chemotaxis is essential for many biological processes. Much of our understanding of the mechanisms underlying chemotaxis is based on a variety of in vitro assays. We review these assays, dividing them into groups depending on the process used to generate the gradient. We describe how each method works, its strengths and limitations, and provide some information about the kinds of cells that have been studied with each assay.

AB - Chemotaxis is essential for many biological processes. Much of our understanding of the mechanisms underlying chemotaxis is based on a variety of in vitro assays. We review these assays, dividing them into groups depending on the process used to generate the gradient. We describe how each method works, its strengths and limitations, and provide some information about the kinds of cells that have been studied with each assay.

KW - Axon guidance

KW - Boyden

KW - Cell migration

KW - Diffusion

KW - Gradient

KW - Invasion assay

KW - Metastasis

KW - Microfluidics

UR - http://www.scopus.com/inward/record.url?scp=67650862834&partnerID=8YFLogxK

U2 - 10.2174/138620709788681952

DO - 10.2174/138620709788681952

M3 - Review article

C2 - 19601755

AN - SCOPUS:67650862834

SN - 1386-2073

VL - 12

SP - 580

EP - 588

JO - Combinatorial Chemistry and High Throughput Screening

JF - Combinatorial Chemistry and High Throughput Screening

IS - 6

ER -

Assays for eukaryotic cell chemotaxis

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this