A Simple Apparatus for Individual C. elegans Culture

William E. Pittman, Drew B. Sinha, Holly E. Kinser, Nisha S. Patil, Eric S. Terry, Isaac B. Plutzer, Julia Hong, Zachary Pincus

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

We miniaturized standard, solid-phase C. elegans culture conditions to produce a system in which many isolated, individual C. elegans can be housed throughout their lives. This system, the “worm corral,” is compatible with high-resolution brightfield and fluorescent microscopy, allowing imaging of fluorescent transgenes and morphological phenotypes from hatch until death. These culture devices can be constructed on the benchtop with commercially available reagents and standard laboratory equipment, making this an attainable solution for most labs.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages29-45
Number of pages17
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2144
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • C. elegans
  • Culture system
  • Lifespan
  • Longitudinal imaging

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