TY - JOUR
T1 - Ascorbate-induced release of LHRH
T2 - Noradrenergic and opioid modulation
AU - Miller, Brian T.
AU - Cicero, Theodore J.
N1 - Funding Information:
‘Supported in part by grants AA-03539 and AA-07144 from the National Institute on Alcohol Abuse and Alcoholism and DA-03833 from the National Institute on Drug Abuse. 2Current address: Brian T. Miller, Ph.D., University of Texas Medical Branch, Department of Anatomy and Neurosciences, Galveston, TX 77550. “Recipient of Research Scientist Award (DA-00095) from the National Institute on Drug Abuse. Requests for reprints should be addressed to Theodore J. Cicero.
PY - 1987/7
Y1 - 1987/7
N2 - Ascorbic acid caused a calcium-dependent release of luteinizing hormone-releasing hormone (LHRH) from the rat mediobasal hypothalamus in vitro. The ascorbate-induced release of LHRH was effectively blocked by the adrenergic α-receptor blocker phentolamine, but not by the β-receptor blocker propranolol. The effect of ascorbate was also suppressed by the mu receptor selective opiate agonist sufentanil. These results suggest that the effects of ascorbic acid on the in vitro release of LHRH are mediated by endogenous norepinephrine. Moreover, our results are consistent with the hypothesis that the release of LHRH in the rat is regulated by the hypothalamic noradrenergic system, which is in turn modulated in some, as yet undetermined, fashion by opioid peptides.
AB - Ascorbic acid caused a calcium-dependent release of luteinizing hormone-releasing hormone (LHRH) from the rat mediobasal hypothalamus in vitro. The ascorbate-induced release of LHRH was effectively blocked by the adrenergic α-receptor blocker phentolamine, but not by the β-receptor blocker propranolol. The effect of ascorbate was also suppressed by the mu receptor selective opiate agonist sufentanil. These results suggest that the effects of ascorbic acid on the in vitro release of LHRH are mediated by endogenous norepinephrine. Moreover, our results are consistent with the hypothesis that the release of LHRH in the rat is regulated by the hypothalamic noradrenergic system, which is in turn modulated in some, as yet undetermined, fashion by opioid peptides.
KW - Ascorbic acid
KW - Endogenous opioid peptides
KW - Hypothalamus
KW - LHRH
KW - Noradrenergic receptors
KW - Noradrenergic system
KW - Opioid receptors
UR - http://www.scopus.com/inward/record.url?scp=0023585032&partnerID=8YFLogxK
U2 - 10.1016/0361-9230(87)90171-7
DO - 10.1016/0361-9230(87)90171-7
M3 - Article
C2 - 2820553
AN - SCOPUS:0023585032
VL - 19
SP - 95
EP - 99
JO - Brain Research Bulletin
JF - Brain Research Bulletin
SN - 0361-9230
IS - 1
ER -