Array biosensor for detection of biohazards

Chris A. Rowe-Taitt, Joel P. Golden, Mark J. Feldstein, John J. Cras, Karen E. Hoffman, Frances S. Ligler

Research output: Contribution to journalArticle

150 Scopus citations

Abstract

A fluorescence-based biosensor has been developed for simultaneous analysis of multiple samples for multiple biohazardous agents. A patterned array of antibodies immobilized on the surface of a planar waveguide is used to capture antigen present in samples; bound analyte is then quantified by means of fluorescent tracer antibodies. Upon excitation of the fluorophore by a small diode laser, a CCD camera detects the pattern of fluorescent antibody:antigen complexes on the waveguide surface. Image analysis software correlates the position of fluorescent signals with the identity of the analyte. This array biosensor has been used to detect toxins, toxoids, and killed or non-pathogenic (vaccine) strains of pathogenic bacteria. Limits of detection in the mid-ng/ml range (toxins and toxoids) and in the 103-106 cfu/ml range (bacterial analytes) were achieved with a facile 14-min off-line assay. In addition, a fluidics and imaging system has been developed which allows automated detection of staphylococcal enterotoxin B (SEB) in the low ng/ml range. Copyright (C) 2000 Elsevier Science S.A.

Original languageEnglish
Pages (from-to)785-794
Number of pages10
JournalBiosensors and Bioelectronics
Volume14
Issue number10-11
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

Keywords

  • Array biosensor
  • Multianalyte detection
  • Planar waveguides

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    Rowe-Taitt, C. A., Golden, J. P., Feldstein, M. J., Cras, J. J., Hoffman, K. E., & Ligler, F. S. (2000). Array biosensor for detection of biohazards. Biosensors and Bioelectronics, 14(10-11), 785-794. https://doi.org/10.1016/S0956-5663(99)00052-4