Arp2/3-independent assembly of actin by Vibrio type III effector VopL

Amy D.B. Liverman, Hui Chun Cheng, Jennifer E. Trosky, Daisy W. Leung, Melanie L. Yarbrough, Dara L. Burdette, Michael K. Rosen, Kim Orth

Research output: Contribution to journalArticle

114 Scopus citations

Abstract

Microbial pathogens use a variety of mechanisms to disrupt the actin cytoskeleton during infection. Vibrio parahaemolyticus (V. para) is a Gram-negative bacterium that causes gastroenteritis, and new pandemic strains are emerging throughout the world. Analysis of the V. para genome revealed a type III secretion system effector, VopL, encoding three Wiskott-Aldrich homology 2 domains that are interspersed with three proline-rich motifs. Infection of HeLa cells with V. para induces the formation of long actin fibers in a VopL-dependent manner. Transfection of VopL promotes the assembly of actin stress fibers. In vitro, recombinant VopL potently induces assembly of actin filaments that grow at their barbed ends, independent of eukaryotic factors. Vibrio VopL is predicted to be a bacterial virulence factor that disrupts actin homeostasis during an enteric infection of the host.

Original languageEnglish
Pages (from-to)17117-17122
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number43
DOIs
StatePublished - Oct 23 2007
Externally publishedYes

Keywords

  • Actin assembly
  • Microbial pathogenesis
  • Stress fibers
  • Virulence
  • WH2 domains

Fingerprint Dive into the research topics of 'Arp2/3-independent assembly of actin by Vibrio type III effector VopL'. Together they form a unique fingerprint.

  • Cite this