@article{bb5d064e3c22444a8f1cd98d41ad3df8,
title = "Aromatic carbohydrate amphiphile disrupts cancer spheroids and prevents relapse",
abstract = "Spheroids recapitulate the organization, heterogeneity and microenvironment of solid tumors. Herein, we targeted spatiotemporally the accelerated metabolism of proliferative cells located on the spheroid surface that ensure structure maintenance and/or growth. We demonstrate that phosphorylated carbohydrate amphiphile acts as a potent antimetabolite due to glycolysis inhibition and to in situ formation of supramolecular net around spheroid surface where alkaline phosphatase is overexpressed. The efficiency of the treatment is higher in spheroids as compared to the conventional 2D cultures because of the 2-fold higher expression of glucose transporter 1 (GLUT1). Moreover, treated spheroids do not undergo following relapse.",
author = "Alexandra Brito and Pereira, {Patr{\'i}cia M.R.} and Reis, {Rui L.} and Ulijn, {Rein V.} and Lewis, {Jason S.} and Pires, {Ricardo A.} and Iva Pashkuleva",
note = "Funding Information: This work was supported by the EU{\textquoteright}s H2020 program (Forecast 668983), the Portuguese Foundation for Science and Technology (grants BD/113794/2015; PTDC/NAN-MAT/28468/ 2017 and PTDC/BTM-MAT/28327/2017), the US Army Research Laboratory and US Army Research Office (contract/grant W911NF-16-1-0113), NSF (grant 1808143), and the research infrastructure support of the NSF CREST Center IDEALS (grant 1547830). AB thanks to the Portuguese league against cancer for her fellowship. The authors also acknowledge the Radiochemistry and Molecular Imaging Probe Core which was supported by NIH grant P30 CA08748. This study was supported in part NIH NCI R35 CA232130 (to J. S. Lewis). PMRP acknowledges the Tow Foundation Postdoctoral Fellowship from the MSKCC Center for Molecular Imaging and Nanotechnology and the Alan and Sandra Gerry Metastasis and Tumor Ecosystems Center of MSKCC. We also would like to acknowledge Komal Mandleywala for her help in the preparation of spheroid slides for immunocytochemistry. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",
year = "2020",
month = oct,
day = "7",
doi = "10.1039/d0nr05008c",
language = "English",
volume = "12",
pages = "19088--19092",
journal = "Nanoscale",
issn = "2040-3364",
number = "37",
}