TY - JOUR
T1 - Armillariella mellea induces maturation of human dendritic cells without induction of cytokine expression
AU - Kim, Sun Kyung
AU - Im, Jintaek
AU - Yun, Cheol Heui
AU - Son, Jin Young
AU - Son, Chang Gue
AU - Park, Dong Ki
AU - Han, Seung Hyun
N1 - Funding Information:
This study was supported by grants from the Oriental Medicine R&D Project (B050018), and the Ministry of Health & Welfare and the Agricultural R&D Promotion Center (107092-03-1-HD120), Republic of Korea.
PY - 2008/9/2
Y1 - 2008/9/2
N2 - Ethnopharmacological relevance: Armillariella mellea, an edible and medicinal mushroom possessing immuno-modulating potential, has been frequently used for the treatment of infectious diseases or cancers. Aim of the study: In order to elucidate immune-regulatory mechanisms of Armillariella mellea, we investigated the effect of water-soluble components from Armillariella mellea (AME) on the regulation of human dendritic cell (DC) maturation and activation. Materials and Methods: Immature DCs (iDCs) were prepared by differentiating human peripheral blood CD14-positive cells with GM-CSF and IL-4. Then, iDCs were treated with AME at 2-20 μg/ml for 48 h and subjected to flow cytometry to analyze the expression of DC markers. Dextran-FITC uptake assay and enzyme-linked immunosorbent assay were performed to examine the endocytic capacity of AME-stimulated DC and their production of cytokines, respectively. Results: iDCs stimulated with AME showed representative features during DC maturation such as up-regulated expression of CD80, CD83, CD86, both MHC class I and II molecules, and CD205, with a simultaneous decrease in the expression of CD206 and the endocytic capacity. Interestingly, AME was not able to induce the production of TNF-α, IL-12p40, or IL-10, whereas lipopolysaccharides induced a substantial increase of all of the cytokines. Conclusion: Armillariella mellea induces maturation of human DCs through a unique mechanism without inducing cytokine expression.
AB - Ethnopharmacological relevance: Armillariella mellea, an edible and medicinal mushroom possessing immuno-modulating potential, has been frequently used for the treatment of infectious diseases or cancers. Aim of the study: In order to elucidate immune-regulatory mechanisms of Armillariella mellea, we investigated the effect of water-soluble components from Armillariella mellea (AME) on the regulation of human dendritic cell (DC) maturation and activation. Materials and Methods: Immature DCs (iDCs) were prepared by differentiating human peripheral blood CD14-positive cells with GM-CSF and IL-4. Then, iDCs were treated with AME at 2-20 μg/ml for 48 h and subjected to flow cytometry to analyze the expression of DC markers. Dextran-FITC uptake assay and enzyme-linked immunosorbent assay were performed to examine the endocytic capacity of AME-stimulated DC and their production of cytokines, respectively. Results: iDCs stimulated with AME showed representative features during DC maturation such as up-regulated expression of CD80, CD83, CD86, both MHC class I and II molecules, and CD205, with a simultaneous decrease in the expression of CD206 and the endocytic capacity. Interestingly, AME was not able to induce the production of TNF-α, IL-12p40, or IL-10, whereas lipopolysaccharides induced a substantial increase of all of the cytokines. Conclusion: Armillariella mellea induces maturation of human DCs through a unique mechanism without inducing cytokine expression.
KW - Armillariella mellea
KW - Dendritic cells
KW - Innate immunity
KW - Mushroom
UR - http://www.scopus.com/inward/record.url?scp=49349110653&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2008.06.032
DO - 10.1016/j.jep.2008.06.032
M3 - Article
C2 - 18640252
AN - SCOPUS:49349110653
SN - 0378-8741
VL - 119
SP - 153
EP - 159
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
IS - 1
ER -