ARCD-1, an apobec-1-related cytidine deaminase, exerts a dominant negative effect on C to U RNA editing

Shrikant Anant, Debnath Mukhopadhyay, Vakadappu Sankaranand, Susan Kennedy, Jeffrey O. Henderson, Nicholas O. Davidson

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Mammalian apolipoprotein B (apoB) C to U RNA editing is catalyzed by a multicomponent holoenzyme containing a single catalytic subunit, apobec-1. We have characterized an apobec-1 homologue, ARCD-1, located on chromosome 6p21.1, and determined its role in apoB mRNA editing. ARCD-1 mRNA is ubiquitously expressed; phylogenetic analysis reveals it to be a distant member of the RNA editing family. Recombinant ARCD-1 demonstrates cytidine deaminase and apoB RNA binding activity but does not catalyze C to U RNA editing, either in vitro or in vivo. Although not competent itself to mediate deamination of apoB mRNA, ARCD-1 inhibits apobec-l-mediated C to U RNA editing. ARCD-1 interacts and heterodimerizes with both apobec-1 and apobec-1 complementation factor (ACF) and localizes to both the nucleus and cytoplasm of transfected cells. Together, the data suggest that ARCD-1 is a novel cytidine deaminase that interacts with apobec-1 and ACF to inhibit apoB mRNA editing, possibly through interaction with other protein components of the apoB RNA editing holoenzyme.

Original languageEnglish
Pages (from-to)C1904-C1916
JournalAmerican Journal of Physiology - Cell Physiology
Volume281
Issue number6 50-6
DOIs
StatePublished - 2001

Keywords

  • Gene cluster
  • Immunofluorescence
  • Protein-protein interaction
  • RNA-protein interaction

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