Arbitrarily primed PCR DNA fingerprinting of Escherichia coli O157:H7 strains by using templates from boiled cultures

G. Madico, N. S. Akopyants, D. E. Berg

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Arbitrarily primed PCR allows genetically different bacterial strains to be distinguished with great sensitivity and efficiency. We report that informative, reproducible arbitrarily primed PCR profiles can be obtained from Escherichia coli O157:H7 strains by using boiled stationary-phase cultures, without the need for time-consuming phenol extraction. This simple template preparation procedure should be especially useful in large epidemiologic studies when many strains most be typed.

Original languageEnglish
Pages (from-to)1534-1536
Number of pages3
JournalJournal of clinical microbiology
Volume33
Issue number6
StatePublished - Jan 1 1995

Fingerprint Dive into the research topics of 'Arbitrarily primed PCR DNA fingerprinting of Escherichia coli O157:H7 strains by using templates from boiled cultures'. Together they form a unique fingerprint.

  • Cite this